《环境昆虫学报》

作者	单位
李冬桂，彭晨，马睿馨，陈亚平，桂富荣，孙仲享	云南农业大学植物保护学院，云南生物资源保护与利用国家重点实验室，昆明 650201

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中文摘要:

为明确虫螨腈对番茄潜叶蛾Tuta absoluta亲代（F0）及子一代（F1）的亚致死效应，以及探究番茄潜叶蛾F0和F1中解毒酶和保护酶系对亚致死浓度虫螨腈的响应情况，本研究采用浸叶法测定虫螨腈对番茄潜叶蛾的毒力，通过生物测定分析了亚致死浓度虫螨腈LC25对番茄潜叶蛾F0和F1生长发育的影响，并测定了虫螨腈LC25浓度处理后番茄潜叶蛾体内的解毒酶系和保护酶系的活力变化。研究发现，虫螨腈对番茄潜叶蛾2龄幼虫的生物活性较高，LC50为1.33 mg/L。亚致死浓度虫螨腈LC25处理后番茄潜叶蛾F0产卵量显著下降，F1的蛹重显著降低，蛹期和成虫前期显著延长。在解毒酶方面，亚致死浓度的虫螨腈对番茄潜叶蛾F0的谷胱甘肽-S-转移酶（GST）和多功能氧化酶（MFO）表现为抑制作用，羧酸酯酶（CarE）没有明显变化；虫螨腈LC25浓度对F1的GST和MFO有诱导作用，其中GST诱导作用最强，而CarE作用不明显。在保护酶方面，亚致死浓度的虫螨腈仅对F0番茄潜叶蛾过氧化氢酶（CAT）有诱导作用，而超氧化物歧化酶（SOD）和过氧化物酶（POD）无变化；虫螨腈LC25浓度对F1的CAT、POD和SOD作用不明显。虫螨腈LC25 浓度显著影响番茄潜叶蛾的生长发育和繁殖，且能显著影响其体内部分解毒酶和保护酶的活性。因此，本研究为促进合理使用虫螨腈防治番茄潜叶蛾提供了有价值的参考。

英文摘要:

In order to evaluate the sublethal effects of chlorfenapyr on F0 generation and progeny (F1 generation) of Tuta absoluta and to explore the response of detoxification enzymes and protective enzymes in F0 and F1 of T. absoluta to chlorfenapyr. This study used the leaf soaking method to determine the toxicity of chlorfenapyr to T. absoluta, and the sublethal effects of LC25 exposure to chlorfenapyr on growth and development of F0 and F1 generations of T. absoluta were analyzed by bioassay and the changes on F0 generation and progeny (F1 generation) of T. absoluta of detoxification enzymes and protective enzymes in the body after LC25 treatment were determined. The results showed that the activity of chlorfenapyr to the 2nd instar larvae of T. absoluta was high with the LC50 value of 1.33 mg/L. The number of eggs laid in the sublethal pesticide concentrations LC25 chlorfenapyr significantly reduced of F0 generation. Exposure to sublethal pesticide concentrations reduced the weight of F1 pupae, significantly prolonged the pupal period and total pre-oviposition period. In terms of detoxification enzymes, there was no significant change in carboxylesterase (CarE) activity, but glutathione S-transferase activity (GSTs) and Mixed-function oxidase (MFO) were distinctly inhibited by sublethal concentrations of chlorfenapyr on F0 generation. There was no significant change in CarE activity compared with the control, but GSTs and MFO were distinctly increased by sublethal concentrations of chlorfenapyr on F1. In terms of protective enzymes, the sublethal concentration of chlorfenapyr had no significant affect on the activities of POD and SOD on F0 generation in T. absoluta, while there was inducedeffect on the activity of CAT and the three protective enzymes CAT, POD and SOD on F0 generation. In summary, chlorfenapyr at the sublethal concentrations could significantly affect the growth and development of T. absoluta and the activity of some detoxification enzymes and protective enzymes in vivo. Therefore, this study provided valuable information for promoting the rational use of chlorfenapyr for controlling T. absoluta.

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