李小雨,胡蝶,黄廷莉,姚洪渭,蒋彩英,2024,二化螟盘绒茧蜂茧丝纤维的形态结构与理化性能[J].环境昆虫学报,46(5):1255-1260 |
二化螟盘绒茧蜂茧丝纤维的形态结构与理化性能 |
Morphological structure and physicochemical properties of silk fibers from the cocoons of the parasitoid wasp, Cotesia chilonis (Matsumura) (Hymenoptera: Braconidae) |
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DOI: |
中文关键词: 二化螟盘绒茧蜂 茧 丝纤维 形态结构 理化性能 |
英文关键词:Cotesia chilonis (Matsumura) cocoon silk fibers morphological structure physicochemical properties |
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中文摘要: |
本文通过扫描电镜(SEM)、氨基酸分析、傅立叶红外光谱(FTIR)、X射线晶体衍射(XRD)和热重分析(TG)等技术对二化螟盘绒茧蜂Cotesia chilonis茧丝纤维的微观形态、氨基酸类别与组成、二级结构和热稳定性等作了分析。结果表明,二化螟盘绒茧蜂的茧丝纤维表面较为粗糙,直径为1.89 ± 0.04 μm。茧丝纤维主要由碳(60.67%)、氮(21.17%)和氧(17.22%)等元素组成,其丝蛋白主要成分包括天冬氨酸/天冬酰胺、丝氨酸和丙氨酸等;蛋白二级结构主要为β-折叠;其峰值降解温度为332.27℃ ± 2.58℃。本文研究揭示了二化螟盘绒茧蜂茧丝纤维的结构特征与理化性能,为拓展该蜂的应用领域、开发性能优越的非蚕丝蛋白以及设计改造新型丝蛋白纤维提供基础。 |
英文摘要: |
In this paper, the morphological structure, amino acid composition, secondary structure and thermal stability of silk fibers from the cocoons of the parasitoid wasp, Cotesia chilonis (Matsumura) (Hymenoptera: Braconidae) were analyzed by the scanning electron microscopy (SEM), amino acid analysis, Fourier transform infrared spectroscopy (FTIR), X-ray crystallography (XRD) and thermogravimetric analysis (TG). The results showed that the surface of the silk fiber from C. chilonis cocoons was relatively rough, and its diameter was 1.89 ± 0.04 μm. It was mainly composed of elements such as carbon (60.67%), nitrogen (21.17%), and oxygen (17.22%). The protein components of C. chilonis cocoons mainly included aspartic acid/asparagine, serine and alanine. The secondary structure of silk proteins from C. chilonis cocoons was mainly β-sheet, and its peak degradation temperature was 332.27 ± 2.58℃. This study reveals the ultrastructural characteristics and physicochemical properties of silk fibers from C. chilonis cocoons, which provides a basis for expanding the application field of C. chilonis, developing non-worm silk proteins with superior performance, and designing and transforming new silk protein fibers. |
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