杨洁,尹伊秀娟,王丽娟,李国平,李裕展,徐建欣,高冠群,詹儒林,王姝逸,2024,非寄主植物精油对桔小实蝇的驱避效果研究[J].环境昆虫学报,(5):1213-1223
非寄主植物精油对桔小实蝇的驱避效果研究
Repellent effect of essential oil from non-host plants against Bactrocera dorsalis
  
DOI:
中文关键词:  桔小实蝇  精油  非寄主植物  产卵驱避  驱避持效期
英文关键词:Bactrocera dorsalis  essential oil  non-host plant  oviposition repellent  duration of repellent holding
基金项目:海南省自然科学基金(321QN0941);三亚市科技创新专项项目(2022KJCX20)
作者单位
杨洁,尹伊秀娟,王丽娟,李国平,李裕展,徐建欣,高冠群,詹儒林,王姝逸 1. 中国热带农业科学院湛江实验站广东湛江5240132. 中国热带农业科学院三亚研究院海南三亚5720243. 天津市农业科学院信息研究所天津 3001924. 天津农学院园艺园林学院天津 3003845. 云南农业大学热带作物学院云南普洱6650996. 中国热带农业科学院南亚热带作物研究所广东湛江5240917. 天津市农业发展服务中心天津 300061 
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中文摘要:
      桔小实蝇Bactrocera dorsalis Hendel幼虫期隐蔽性的取食习性降低了化学防治的效果,而普遍存在的杀虫剂耐药性则加剧了这一问题。利用非寄主植物挥发物驱避害虫是替代化学防治、有效控制虫害的策略之一。为了筛选更有效的植物源产卵驱避剂,本研究选取23种非寄主植物精油作为候选材料,进行4轮试验,首先采用行为趋向反应试验,筛选出8种驱避率较高且选择反应率和驱避反应率差异极显著(P<0.01)的非寄主植物精油:绿花白千层、山鸡椒、广藿香、当归、茶树、茴香、薄荷、艾草;然后通过产卵驱避活性试验,筛选出4种驱避率高且处理组与对照组的产卵量差异极显著(P<0.01)的植物精油:绿花白千层、广藿香、当归、茶树。浓度梯度试验表明,浓度为5 000 μg/mL时,绿花白千层、广藿香、当归驱避率最高,浓度为3 000 μg/mL时,茶树精油驱避率最高;在最佳浓度下对桔小实蝇产卵驱避持效性分析,结果表明最佳浓度下4种精油处理过的果实,随着时间延长,桔小实蝇产卵孔和产卵量逐渐增加,驱避率逐渐降低,驱避产卵活性逐渐消失,前3 d降速较快,然后逐渐放缓。本研究通过行为趋向反应试验、产卵驱避活性试验、浓度梯度试验和驱避活性持效试验,从23种非寄主植物精油中,筛选到绿花白千层精油对桔小实蝇有较高的产卵驱避活性,通过剂型改良等措施有望开发成植物源驱避剂。
英文摘要:
      The hidden feeding habits of Bactrocera dorsalis in larval stage reduce the efficiency of chemical control, which is exacerbated by the widespread insecticide resistance. In order to develop more fertile repellent plant sources, four rounds of experiments were conducted on 23 non-host plant essential oils selectedas candidate materials. First, eight non-host essential oils with high repellent rate and significant difference between selection response rate and repellent response rate (P<0.01) were selected by behavioral tactic response experiment, which included Melaleuca viridiflora, Litsea cubeba, Pogostemon cablin, Angelica sinensis, Melaleuca alternifolia, Foeniculum vulgare, Mentha canadensis, Artemisia argyi. Then, through the oviposition repellent activity experiment, four plant essential oils with high repellent rate and extremely significant difference (P<0.01) between the treatment group and the control group were further screened. Then through the concentration gradient experiment, the repellent rate of M.viridiflora, P.cablin, A. sinensiswas the highest when the concentration was 5 000 μg/mL. When the concentration was 3 000 μg/mL, the repellent rate of M. alternifolia essential oil was the highest. Finally, the oviposition repellent effect of B. dorsalis was analyzed at the optimal concentration. The results showed that the oviposition holes and oviposition amount of B. dorsaliswere increased gradually, the repellent rate was decreased gradually, and the repellent oviposition activity of B. dorsalis was disappeared gradually with the propioning time of the fruits treated with the four essential oils at the optimal concentration. The rate of decline was faster in the first 3 days and then slowed down gradually. On the whole, M. viridiflora essential oil shows better performance in all aspects, and have higher oviposition repellent activity against B. dorsalis. It is expected to be developed into plant repellent agents in the future through the improvement measures.
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