《环境昆虫学报》

为寻找对草地贪夜蛾Spodoptera frugiperda具有良好杀虫活性的化学成分，对蔓长春花石油醚、三氯甲烷、乙酸乙酯和水萃取物通过小叶碟添加法、浸叶法和浸虫法，测定其拒食、胃毒和触杀活性。结果表明：蔓长春花石油醚萃取物对草地贪夜蛾3龄幼虫的触杀活性最好，第1、3、5和7天的LC50分别为6.11、5.43、5.27和4.62 mg/mL。采用硅胶柱层析对蔓长春花石油醚萃取物进行分离，得到22个馏分，馏分5和馏分7的触杀活性最好。对馏分5进行GC-MS分析，主要化学成分为对二甲苯（17.56%）、壬烷（20.92%）和十三烷（28.33%）。馏分7经小孔树脂（MCI）分离后得到8个馏分，馏分Ⅵ的触杀活性最好，经GC-MS分析，主要化学成分为壬烷（19.09%）、十三烷（53.19%）和2，2，4，6，6-五甲基庚烷（5.21%）。对二甲苯的触杀活性最好，第1、3、5和7天时LC50分别为10.45、8.87、6.54和5.05 mg/mL。随着对二甲苯浓度的升高，乙酰胆碱酯酶（AChE）和Ca2+-Mg2+-ATPase的活性呈现先激活后抑制的趋势，羧酸酯酶（CarE）的活性呈现先抑制后激活的趋势。

英文摘要:

In order to search for chemical components with good insecticidal activity against Spodoptera frugiperda, antifeedant activity, the stomach poisoning, and contact toxicity of petroleum ether, chloroform, ethyl acetate, and water extracts from Vinca major were determined by small leaf plate addition, leaf soaking, and insect soaking method. The results showed that the petroleum ether extract of V. major had the best contact activity against the S. frugiperda with LC50 values of 6.11, 5.43, 5.27, and 4.62 mg/mL respectively at 1 d, 3 d, 5 d, and 7 d. Component separation of petroleum ether extract from V. major were separated by silica gel column chromatography, 22 fractions were obtained, with fractions 5 and 7 having the best contact activity. Fraction 5 were analyzed by GC-MS. The main chemical components were p-xylene (17.56%), n-nonane (20.92%) and tridecane (28.33%). 8 fractions were obtained from fraction 7 by microporous resin chromatography. Fraction VI had the best contact activity. According to GC-MS analysis, the main chemical components were n-nonane (19.09%), tridecane (53.19%) and 2,2,4,6,6-pentamethylheptane (5.21%). The LC50 of p-xylene was 10.45, 8.87, 6.54 and 5.05 mg/mL at 1 d, 3 d, 5 d and 7 d, respectively. The activities of AChE and Ca2+-Mg2+-ATPase firstly increased and then decreased with the increase of p-xylene concentration. The activities of CarE were firstly inhibited and then activated.

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