| 张冰晨，杨卫诚，肖佳兴，何琴琴，白小节，陈航丹，车冰洁,2024,不同寄主植物对桫椤叶蜂的生长发育及其肠道细菌群落的影响[J].环境昆虫学报,(4):886-896 |
| 不同寄主植物对桫椤叶蜂的生长发育及其肠道细菌群落的影响 |
| Effects of different host plants on the growth, development and gut bacterial community of Rhoptroceros cyatheae larvae |
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| DOI： |
| 中文关键词: 桫椤叶蜂 生长发育 寄主植物 肠道细菌 16S rDNA |
| 英文关键词:Rhoptroceros cyatheae developmental duration host plant gut bacteria 16S rDNA |
| 基金项目:黔林科合〔2022〕27号 |
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| 摘要点击次数: 236 |
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| 中文摘要: |
| 为明确桫椤叶蜂Rhoptroceros cyatheae幼虫取食桫椤Alsophila spinulosa及小黑桫椤Gymnosphaera metteniana var. subglabra两种寄主植物后肠道菌群的结构组成和多样性，及取食两种寄主植物上的平均发育历期，利用Illumina HiSeq技术对取食两种寄主植物后的桫椤叶蜂幼虫肠道细菌的16S rDNA的V3~V4区域进行测序与分析，并记录在寄主植物上的平均发育历期。结果显示：（1）取食小黑桫椤的叶蜂卵历期、老熟幼虫期、蛹期（4.07±0.80 d、9.15±1.36 d和3.82±1.11 d）相比取食桫椤的（4.57±0.65 d、10.75±1.73 d和4.73±0.86 d）显著缩短，成虫寿命无显著影响；（2）取食桫椤的幼虫肠道注释到细菌4门6纲17目26科37属，取食小黑桫椤的桫椤叶蜂幼虫肠道注释到细菌12门17纲32目44科58属；其中，变形菌门Proteobacteria（83.68%±3.74%、81.18%±0.75%）是两组肠道的优势菌门；肠杆菌属Enterobacter（61.98%±10.64%、30.25%±1.75%）为两组的优势菌属。α多样性分析表明取食小黑桫椤的叶蜂幼虫肠道微生物群落多样性略高于取食桫椤的叶蜂幼虫，β多样性分析结果显示，取食小黑桫椤的叶蜂幼虫肠道微生物群落更稳定。KEGG功能预测分析显示取食桫椤和小黑桫椤的叶蜂肠道细菌群落功能无差异，肠道细菌主要参与宿主的营养代谢。本研究为进一步探讨影响桫椤叶蜂肠道细菌变化的因素，以及后续研究肠道细菌与寄主植物之间的互作提供参考。 |
| 英文摘要: |
| To clarify the structural composition and diversity of the gut bacteria of Rhoptroceros cyatheae feeding on Alsophila spinulosa and Gymnosphaera metteniana var. subglabra, and the average developmental epoch on the two host plants. The Illumina HiSeq technology was used to sequence and analyze the V3~V4 regions of 16S rDNA of the gut bacteria of R. cyatheae larvae feeding on the two host plants, and recorded their developmental history. The results showed that: (1) The egg, mature larval and pupal stages (4.07±0.80 d, 9.15±1.36 d and 3.82±1.11 d) of R. cyatheae feeding on G. metteniana var. subglabra were significantly shorter compared with those feeding on A. spinulosa (4.57±0.65 d, 10.75±1.73 d and 4.73±0.86 d). There was no significant effect on the adult lifespan. (2) The larvae gut bacteria of R. cyatheae fed on A. spinulosa had been annotated into 4 phyla, 6 classes, 17 orders, 26 families and 37 genera; the larvae gut bacteria of R. cyatheae fed on G. metteniana var. subglabra had been annoted into 12 phyla, 17 classes, 32 orders, 44 families and 58 genera. The Proteobacteria (83.68%±3.74%, 81.18%±0.75%) was the dominant bacteria in both groups; Enterobacter (61.98%±10.64%, 30.25%±1.75%) was the dominant genus in both groups. The alpha diversity analysis showed that the gut microbial community diversity of R. cyatheae larvae feeding on G. metteniana var. subglabra was slightly higher than that feeding on A. spinulosa. Beta diversity analysis showed that the gut microbial community of R. cyatheae larvae feeding on G. metteniana var. subglabra was more stable. KEGG functional prediction revealed no difference in the function of the gut bacterial community between R. cyatheae larvae feeding on the two host plants, and the gut bacteria were mainly involved in the nutrient metabolism of the host. This should be a case study for the factors that influence gut bacteria of R. cyatheae, and may provide insights into the interactions between gut bacteria and different host plants. This study provides a reference for further exploring the factors affecting the changes of gut bacteria of R. cyatheae and for subsequent studies on the interactions between gut bacteria and host plants. |
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