| 唱通，赵康竹，汪以馼，乔欢欢，刘文彬，闫春财,2023,RNAi技术在昆虫基因组学研究及其生物防控中的研究进展[J].环境昆虫学报,(6):1514-1524 |
| RNAi技术在昆虫基因组学研究及其生物防控中的研究进展 |
| Progress of RNAi technology in insect genomics and biological control |
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| DOI： |
| 中文关键词: RNAi 生物防控 导入途径 害虫控制 基因学研究 |
| 英文关键词:RNAi biological prevention and control import route pest control genetic research |
| 基金项目:国家自然科学基金（32370489，32170473）；组分中药国家重点实验室基金（CBCM2020203） |
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| 中文摘要: |
| RNAi技术是通过将双链RNA（dsRNA）导入宿主细胞，沉默相关基因进而干扰宿主生长发育的一项技术，广泛应用于昆虫的生物防控。近年来，基于RNAi开展昆虫的防控技术研究中发现RNAi的效率受到多方面因素的影响，其中dsRNA的导入途径是关键的影响因素。本文总结了显微注射法、电穿孔介导法、饲喂法、转基因法、纳米载体介导法等5种方法在昆虫的基因组学研究和RNAi生物防控中的应用效果，比较了不同方案的优缺点，以期为昆虫的RNAi应用研究和生物防控技术提供参考。 |
| 英文摘要: |
| RNAi technology is a technique that silences associated genes and interferes with host growth and development by introducing double-stranded RNA (dsRNA) into host cells. This technique is widely used in the biological prevention and control of terrestrial insects. In recent years, researchers have explored RNAi biological control techniques in insects. The efficiency of RNAi is affected by various factors, among which the dsRNA import pathway is the key factor. Here we reviewed the five methods of microinjection, electroporation, feeding, transgenic, nanocarrier mediated application in insect genetics and RNAi biological prevention and control, as well as the advantages and disadvantages of the scheme, in order to provide a reference for RNAi research and pest biological control of insects. |
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