| 余蔓，雷勇辉，孙燕飞，熊杰，李杨,2021,马蜂内生酵母种群结构分析及Metschnikowia pulcherrima的分离和鉴定[J].环境昆虫学报,(5):1288-1294 |
| 马蜂内生酵母种群结构分析及Metschnikowia pulcherrima的分离和鉴定 |
| Population structure analysis of endophytic yeast of Vespa velutina and identification of Metschnikowia pulcherrima |
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| DOI： |
| 中文关键词: 马蜂 内生酵母 高通量测序 26S rDNA |
| 英文关键词:Vespa velutina endophytic yeast high-throughput sequencing 26S rDNA |
| 基金项目:国家自然科学基金（31860003） |
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| 中文摘要: |
| 本文以新疆石河子西公园的马蜂Vespa velutina为研究对象，结合基于26S rDNA的高通量测序技术和稀释平板分离的方法，研究马蜂内生酵母物种组成及其多样性。结果显示，马蜂体内包含Cutaneotrichosporon、Torulaspora、Pichia等10个属内生酵母；含有Cutaneotrichosporon cutaneum、Torulaspora delbrueckii、Sporidiobolales sp.等10种内生酵母。Shannon指数、Simpson指数、ACE指数和Chao1指数分别为0.619215、0.681265、84.38456和76。利用稀释平板分离方法分离获得一株内生酵母，结合菌落和细胞形态观察、生理生化检测以及26S rDNA分子鉴定等方法，该酵母菌株为美极梅奇酵母Metschnikowia pulcherrima。本研究可为后续的内生酵母菌功能研究及酵母菌资源开发与利用奠定基础。 |
| 英文摘要: |
| The composition and diversity of the endophytic yeast species in Vespa velutina Smith were studied by using high-throughput sequencing based on 26S rDNA and diluted plate method in Shihezi West Park, Xinjiang. The results showed that there were ten genus including Cutaneotrichosporon, Torulaspora, etc. There were ten species of endophytic yeast including Cutaneotrichosporon cutaneum, Torulaspora delbrueckii, Sporidiobolales sp., etc. Shannon index, Simpson index, ACE index and Chao 1 Index are 0.619215，0.681265，84.38456 and 76 respectively. A strain of endophytic yeast, Metschnikowia pulcherrima, was isolated by using diluted plate method combined with colony and cell morphology observation, physiological and biochemical detection and 26S rDNA molecular identification. This study can lay the foundation for subsequent study on endophytic yeast function and utilization of yeast resources. |
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