《环境昆虫学报》

为探索拟黄花乌头 Aconitum anthoroideum DC. 作为生物农药的应用价值，以拟黄花乌头整株为研究对象，在活性跟踪的基础上，综合应用硅胶柱层析、ODS、Sephadex LH-20等方法对拟黄花乌头甲醇粗提物进行分离纯化，从中分离得到了4个生物碱类化合物，分别为4-hydroxynicotinic acid methyl ester (1)、Ranaconitine (2)、Lappaconitine (3)、13-hydroxylappaconitine (4)，其中4-hydroxynicotinic acid methyl ester为首次从天然产物中分离得到。采用点滴法测定4个化合物对褐飞虱 Nilaparvata lugens Stal和白背飞虱Sogatella furcifera Horvath的触杀活性，结果表明4种化合物对褐飞虱和白背飞虱具有显著的触杀活性，处理后48 h的LD50 分别为0.26~0.38 μg/头、0.25~0.33 μg/头。研究结果为进一步研究拟黄花乌头中的杀虫活性成分提供基础数据。

英文摘要:

In order to explore the application value of Aconitum anthoroideum DC. as biological pesticides，4-hydroxynicotinic acid methyl ester (1), which is described herein as a natural product for the first time, with three norditerpenoid alkaloids constituents, Ranaconitine (2), Lappaconitine (3), 13-hydroxylappaconitine (4), were isolated from the methanol extract of the whole plant of A. anthoroideum followed by the activity guided method with combination of column chromatography of silica gel, ODS, Sephadex LH 20, etc. Compounds 1 to 4 exhibited activities with LD50 values for 48 h ranging from 0.26 to 0.38 μg/insect against Nilaparvata lugens and from 0.25 to 0.33 μg/insect against Sogatella furcifera by drop method, respectively. The results provided a theoretical basis for further research on the insecticidal activity of natural products of A.anthoroideum.

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