路伟,李佳琪,邝鹏昆,肖绍伟,王茂源,郭永超,徐汉虹,2017,印楝素A和印楝素B对棉铃虫生长发育的影响[J].环境昆虫学报,(4):945-959 |
印楝素A和印楝素B对棉铃虫生长发育的影响 |
Effects ofazadirachtin A and azadirachtin B on the growth and development of Heliothis armigera |
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DOI: |
中文关键词: 印楝素A 印楝素B 棉铃虫 生物活性 作用机理 |
英文关键词:Azadirachtin A azadirachtin B Helicoverpa armigera bioactivity mechanism |
基金项目:新疆农业大学大学生创新项目(DXSCX92016001) |
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中文摘要: |
为了明确印楝素A和印楝素B生物活性的差异,选用印楝素A和印楝素B对棉铃虫生长发育的影响进行了比较研究。结果表明:印楝素A和印楝素B对棉铃虫3龄幼虫具有良好的拒食活性,5 μg/mL处理48 h对棉铃虫3龄幼虫的拒食率分别为85.17%和69.02%。分别用含有药剂(1 μg/mL)的饲料饲喂棉铃虫5龄幼虫,结果表明:印楝素A和印楝素B能够明显抑制棉铃虫5龄幼虫的体重增长,处理14 d后幼虫的体重分别下降50.28%和43.08%,仅有少量个体化蛹,化蛹率分别为26.67%和13.33%。进一步的羽化结果表明:经印楝素A和印楝素B处理的虫蛹均未能完成羽化。综合各阶段试验结果来看,印楝素A和印楝素B的生物活性存在差异,印楝素B对棉铃虫生长发育的抑制作用高于印楝素A。 |
英文摘要: |
In order to confirm the bioactivity differences, azadirachtin A and azdirachtin B were used to study the effects on growth and development of Helicoverpa armigera. The results showed that: Azadirachtin A and azadirachtin B revealed favorable antifeedant activity against 3rd instar larvae of H.armigera, when the 3rd instar larvae of H.armigera were treated with 5 μg/mL of azadirachtin A and azadirachtin B for 48 h, the antifeedant rates were 85.17% and 69.02%. The growing development results showed that azadirachtin A and azadirachtin B can inhibit the growth of 5th instar larvae of H.armigera after 14 d at 5 μg/mL, and larval weight were decreased by 50.28% and 43.08%, respectively. Only a few individuals completed pupation, pupation rate were 26.67% and 13.33%. The further results showed that the pupae of H.armigera treated by azadirachtin A and azadirachtin B were failed to complete eclosion. According to the test results at various stages, the conclusion was that the bioactivities of azadirachtin A and azadirachtin B were different, and inhibition of azadirachtin B on the growth of H.armigera was higher than azadirachtin A. |
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