| 杨佳琪，檀军，曹米兰，金道超，郭建军*,2017,CCK-8法检测九香虫血淋巴对人乳腺癌MCF-7细胞增殖的抑制作用[J].环境昆虫学报,39(1):193-197 |
| CCK-8法检测九香虫血淋巴对人乳腺癌MCF-7细胞增殖的抑制作用 |
| The inhibitory effect of haemolymph fromAspongopus chinensis Dallas on growth of human breast cancer MCF-7 by CCK-8 |
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| DOI： |
| 中文关键词: 九香虫 血淋巴 乳腺癌 CCK-8法 |
| 英文关键词:Aspongopus chinensis Dallas haemolymph breast cancer CCK-8 |
| 基金项目:国家自然科学基金（81360612）；贵州省科学基金省校联合项目（黔科合LH字\[2014\]7650）；贵州省优秀青年科技人才培养对象专项资金项目（黔科合人字\[2015\]25号）；贵州省科技厅创新人才团队建设项目（黔科合人才团队\[2014\]4001号 |
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| 中文摘要: |
| 为了明确九香虫血淋巴对人乳腺癌MCF-7细胞增殖的抑制作用，本文利用Bradford法检测九香虫血淋巴浓度，用不同浓度的九香虫血淋巴处理体外培养的MCF-7细胞，采用CCK-8法检测九香虫血淋巴对MCF-7细胞的抑制作用。结果表明：九香虫血淋巴能显著抑制人乳腺癌MCF-7细胞的生长，且对MCF-7的抑制率呈时间、剂量依赖关系。 |
| 英文摘要: |
| To clear the inhibitory effect on human breast cancer cell line MCF-7 by the haemolymph from Aspongopus chinensis Dallas, 1851, Bradford method was used to test the concentrations of the haemolymph from A. chinensis, the MCF-7 cells cultured in vitro were treated by different concentrations of the haemolymph, and at last the inhibition rates to MCF-7 cells were detected by CCK-8 method. The results showed that the haemolymph from A. chinensis could inhibit proliferation of human breast cancer MCF-7 cells in vitro with dosage- and time-dependent effects. |
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