| 张志建，郑长英，赵川德，万方浩，王俊平*,2017,利用冷冻切片对球孢白僵菌侵染西花蓟马过程的观察[J].环境昆虫学报,39(1):181-186 |
| 利用冷冻切片对球孢白僵菌侵染西花蓟马过程的观察 |
| Microsc opicobservations of the process of Beauveria bassiana infecting Frankliniella occidentalis through frozen sections |
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| DOI： |
| 中文关键词: 西花蓟马 球孢白僵菌 冷冻切片 侵染 组织病理变化 |
| 英文关键词:Frankliniella occidentalis Beauveria bassiana frozen sections infect histopathological changes |
| 基金项目:国家自然科学基金（31201777）；青岛农业大学高层次人才科研基金（630609）；山东省“泰山学者”建设工程专项经费；青岛农业大学应用型人才培养特色名校建设工程大学生科技创新项目 |
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| 中文摘要: |
| 为探索球孢白僵菌对西花蓟马的侵染，研究球孢白僵菌在西花蓟马体表和虫体内的侵染过程，采用冷冻切片与HE染色技术，对被球孢白僵菌侵染的西花蓟马成虫进行组织切片及HE染色。结果表明，接菌4 h,体表粘附的孢子开始萌发，8-12 h芽管继续伸长并出现穿透体壁。18 h虫菌体出现在西花蓟马体内，球孢白僵菌成功侵入西花蓟马体内。之后，西花蓟马体内虫菌体、菌丝明显增多，各组织器官均受到感染，消化道、肌肉组织等发生病变。 |
| 英文摘要: |
| The pathological changes of Frankliniella occidentalis infected by Beauveria bassiana were observed using frozen sections with hematoxylin-eosin (H & E) staining. The result showed that the conidia began to germinate which adhesion on the cuticle after inoculated 4 h. Within 8-12 h after inoculation, the germ tubes continued to elongation and appeared penetrating the integument. At 18 h post inoculation, the hyphal bodies succeeded to be observe in the internal tissue and began to reproduce in the hemocoele. And then, the hyphal bodies and hyphae increased, tissues and organs were infected, muscle tissue and alimentary cancal began to undergo pathological changes. |
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