温娟,郅军锐*,吕召云,张羽宇,2017,二斑叶螨为害对番茄叶片主要营养物质和防御酶活性的影响[J].环境昆虫学报,39(1):172-180 |
二斑叶螨为害对番茄叶片主要营养物质和防御酶活性的影响 |
Effects of Tetranychus urticaefeeding on the contents of main nutrient and defensive enzymes activities of tomato leaves |
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DOI: |
中文关键词: 二斑叶螨 番茄 营养物质 防御酶活性 |
英文关键词:Tetranychus urticae tomato nutrients protective enzyme |
基金项目:贵州省普通高等学校系统与应用蜱螨学创新团队(黔教合人才团队字\[2014\]33);贵州省国际合作项目(黔科合外G字\[2011\]7002号);贵州大学研究生创新基金(研农2015033) |
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中文摘要: |
本文研究了二斑叶螨Tetranychus urticae为害对寄主植物番茄营养物质和防御酶活性的影响。结果表明:二斑叶螨为害对番茄植株体内营养物质含量和防御酶活性均有一定影响,且为害密度和为害时间存在交互作用。二斑叶螨为害后番茄叶片可溶性糖含量下降幅度与叶螨为害密度和时间呈正比;可溶性蛋白和游离氨基酸含量在二斑叶螨高密度为害3 d时最低。二斑叶螨为害后番茄叶片防御酶活性普遍增强,过氧化物酶POD活性在5头/叶密度时与为害时间呈正比;苯丙氨酸解氨酶PAL、多酚氧化酶PPO和脂氧合酶LOX活性的变化一致,均在中密度为害时活性最高。以上结果说明番茄叶片通过调节其体内营养物质含量和各种防御酶活性的变化,对二斑叶螨的胁迫产生了应激反应。 |
英文摘要: |
In order to assess the effect of Tetranychus urticae feeding on the host plants, the changes of main nutrients content and protective enzymes activity of tomato were tested after it was infested by T. urticaeat different population densities and attacking durations. The results showed that the density and attacking duration of T. urticae had significant effect on contents of main nutrients and the activities of protective enzyme, and the population densities and attacking durations had interactive effect. The contents of soluble sugar deceased significantly with the increasing mite density and feeding duration. The contents of soluble protein and free amino acid were lowest at 25 mites/leaf at 3 days, but soluble protein content increased at 15 mites/leaf at 2 day, and free amino acid increased at 15 or 25 mites/leaf at 2 days. The activities of defensive enzyme generally increased after infestation of T. urticae. The POD activities of tomato infested by 5 mites/leaf significantly increased with the feeding duration, while the activities of PAL, PPO and LOX were highest at moderate damage. The results indicated that the damage of T. urticae induced tomato resistance and tomato produce response by adjusting the contents of various nutrients and activities of defensive enzyme. |
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