| 颜珣，韩日畴*,2016,生物杀虫剂-昆虫病原线虫的培养技术[J].环境昆虫学报,(5):1044-1051 |
| 生物杀虫剂-昆虫病原线虫的培养技术 |
| Production technology of the bio-insecticides: Entomopathogenic nematodes |
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| DOI： |
| 中文关键词: 昆虫病原线虫 大量培养 昆虫活体培养 体外固体培养 体外液体培养 |
| 英文关键词:Entomopathogenic nematodes mass production in vivo culture in vitro solid culture in vitro liquid culture |
| 基金项目:广东省省级科技计划项目（2014A020208075,2016A020210076）；广东省科学院广州市珠江科技新星专项（2013J2200090）；广东省科学院项目（2016GDASPT-0305，rcjj201201） |
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| 中文摘要: |
| 昆虫病原线虫是新型的生物杀虫剂，对钻蛀及土栖性害虫防效较好，具有部分替代化学农药的潜力。昆虫病原线虫的产业化培养是昆虫病原线虫商业化应用的基础。本文介绍了目前世界上常用的昆虫病原线虫的培养方法，包括活体培养、半固体培养和液体培养的技术，这些技术的应用有助于昆虫病原线虫的种质保存及培养，为拓展昆虫病原线虫的产业化生产和应用奠定了基础。 |
| 英文摘要: |
| Entomopathogenic nematodes are biological control agents that can be used to effectively control boring and subterranean insect pests. Mass production is a prerequisite for the successful commercialisation of entomopathogenic nematodes. The different production techniques, includingin vivo and in vitro methods, are described in the present paper. The application of the production techniques is the basis of nematode stock preservation and mass production, which will explore the industrial production and commercialisation of entomopathogenic nematodes. |
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