《环境昆虫学报》

使用杂鳞库蚊复组CO I 部分序列和ITS 序列构建分子发育树，并基于CO I 序列计算该复组种内和种间的Kimura-two-Parameter ( K2P) 距离，探讨环带库蚊的分类地位和杂鳞库蚊复组内各亲缘种的系统发育关系。环带库蚊和杂鳞库蚊的种间K2P 距离为0.24% － 0.72%，支持“环带库蚊是杂鳞库蚊的同物异名”这一观点; 杂鳞库蚊( 环带库蚊) 和伪杂鳞库蚊、三带喙库蚊的种间K2P 距离为4.41% － 9.68%，同时分子系统树显示各个种分别聚类，互为姐妹群，再次证明三者互为独立的种; 环带库蚊和杂鳞库蚊聚类的分支最接近树的端部，三带喙库蚊分支最接近树的基部，提示三带喙库蚊最早发生分化，而杂鳞库蚊( 环带库蚊) 最晚发生分化; 采集自日本的三带喙库蚊种内K2P 距离为0.48% － 2.68%，而它们与采集自中国、印度的该种K2P 距离为4.17% － 6.76%，日本产三带喙库蚊聚集成一支，并与中印产地的聚类分支互为姐妹群，这些结果提示日本的三带喙库蚊有种下，甚至种级分化的趋势。

英文摘要:

Discussions about the classification status of Culex annulus and the relationship of C. vishnui complex were held with the Kimura-two-Parameter ( K2P) distances based on CO I and phylogenetic trees based on CO I and ITS. The K2P distance between C. annulus and C. vishnui was 0.24% － 0.72%，which supported “C. annulus is a synonym of C. vishnui”. The interspecific K2P distance among C. vishnui ( containing C. annulus) ，C. pseudovishnui and C. tritaeniorhynchus were 4.41% － 9.68%. From the view of the phylogenetic trees，each species respectively gathered and became sister group each other， which demonstrated again their being independent species of one another. C. annulus and C. vishnui being at the top of the phylogenetic trees indicated that they differentiated later，while C. tritaeniorhynchus being at the root indicated that differentiated earlier. The intraspecific K2P distance of C. tritaeniorhynchus mined in Japan was 0.48% － 2.68%，and the interspecific K2P distances among those mined in Japan，China and India was 4.17%－6.76% . Furthermore，according to the phylogenetic trees，C. tritaeniorhynchusfrom Japan formed a branch themselves，then became the sister group with the branch formed by those from China and India. These results implied that Japanese C. tritaeniorhynchus had the trend to differentiate at subspecies or even species level.

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