| 武宇鹏，武春生，陆俊娇，杨静，朱朝东*,2016,苹果园鳞翅目夜蛾科DNA条形码鉴定[J].环境昆虫学报,(4):813-820 |
| 苹果园鳞翅目夜蛾科DNA条形码鉴定 |
| DNA barcoding of moths ( Lepidoptera: Noctuidae) in an apple orchard |
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| DOI： |
| 中文关键词: 果园 夜蛾科 DNA 条形码 |
| 英文关键词:Orchard noctuidae DNA barcoding |
| 基金项目:山西省自然基金项目( 2015011077) ; 太原科技大学博士科研启动基金( 20142031) |
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| 中文摘要: |
| 为了检验DNA 条形码在鳞翅目夜蛾科蛾类鉴定中的可行性，本文对采自北京昌平苹果园内的夜蛾科14 种71 头蛾类标本分别提取了DNA，并扩增了线粒体cox1 及核基因28S，利用系统发育树、遗传距离、阈值等方法进行了鉴定和比较分析。同时，检验了目前BOLD 系统的鉴定成功率。实验表明，基于cox1 基因和BOLD 系统的鉴定成功率达到了100%，而基于28S 则很低，为64.8%。用不同方法构建的系统发育树，鉴定结果均相同。93% 的种内遗传距离小于1%，94%的种间遗传距离为大于3%，种内种间的遗传距离形成明显的3%阈值现象。 |
| 英文摘要: |
| In order to test the feasibility of DNA barcoding in identification of noctuid moths of Lepidoptera，71 samples ( 14 species in total) were collected from an apple orchard of Changping District，Beijing. And the mitochondrial gene of cox1 and the nuclear gene of 28S were amplified and sequenced. The comprehensive analysis was performed with the methods of phylogenetic trees，genetic distance，and
threshold value. Meanwhile，the success rate of BOLD system for identifying species was examined. The results were as follows: With the cox1 gene and the BOLD system，the success rate of identification achieved 100%. However，the fragment of 28S only possessed the success rate of 64.8%. Using different methods of phylogenetic analysis，the topology structures of different phylogeny trees were consistent，as was the case in species identification. The intraspecific distances that below 1% accounted for 93%，and the
interspecific distances that more than 3% occupied 94%. The interspecific genetic distances were greater than the intraspecific distances，with the threshold value of 3%． |
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