| 赵洁，程代凤，陆永跃*,2016,棉花粉蚧化学感受蛋白基因PsCSP10的克隆及表达谱分析[J].环境昆虫学报,(4):728-735 |
| 棉花粉蚧化学感受蛋白基因PsCSP10的克隆及表达谱分析 |
| Molecular cloning and expression analysis of chemosensory protein genePsCSP10 in Phenacoccus solenopsis Tinsley ( Hemiptera: Pseudococcidae) |
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| DOI： |
| 中文关键词: 棉花粉蚧 化学感受蛋白基因 克隆 序列分析 表达谱 |
| 英文关键词:Phenacoccus solenopsis Tinsley chemosensory protein gene molecular cloning sequence
analysis expression profile |
| 基金项目:广东省自然科学基金( S2013010013526) ; 广东省研究生教育创新计划( 2013JDXM14) |
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| 中文摘要: |
| 本研究克隆出了棉花粉蚧Phenacoccus solenopsis Tinsley 化学感受蛋白( CSPs) 基因PsCSP10 的全长cDNA
( GenBank 登录号: KT958555) ，其核苷酸序列长640 bp，编码128 个氨基酸，预测其成熟蛋白分子量14. 99 kD，
等电点6. 61，且含有4 个保守的半胱氨酸，符合昆虫CSPs 的典型特征。该基因编码的氨基酸序列和其他昆虫化学
感受蛋白基因编码的氨基酸序列相似性为50% － 56%。应用Ｒeal-time PCＲ 测定的结果表明棉花粉蚧各发育阶段中
PsCSP10 均有表达，但在雄成虫中的相对表达量显著高于其他发育阶段。在分别用扶桑Hibiscus rosa － sinensis L. 、
棉花Gossypium hirsutum L. 、马缨丹Lantana camara L. 饲养获得的雄成虫中PsCSP10 相对表达量不存在差异。研究
结果为进一步明确棉花粉蚧中PsCSP10 的功能奠定了基础。 |
| 英文摘要: |
| In this study，one CSP gene in cotton mealybug Phenacoccus solenopsis Tinsley was cloned and characterized，and was named PsCSP10 (GenBank accession no. : KT958555) . The PsCSP10 cDNA was 640 bp in length，encoding 128 amino acid residues with the predicated molecular mass of approximately 14.99 kD and pI of 6. 61，of which four conservative cysteines were included. The alignment of PsCSP10
showed moderate similarity ( 50% － 56%) with the CSP genes of other insects analyzed with the deduced amino acid sequences. By Ｒeal － time PCＲ analysis，PsCSP10 was revealed to express across various development stages，and the expression level was more in the male adult. The expression of PsCSP10 in the mealybug male adults fed on Chinese rose ( Hibiscus rosa-sinensis L. ) ，cotton (Gossypium hirsutum L. ) and common lantana ( Lantana camara L. ) ，respectively，had not significant difference. The results became
the basis for further research on PsCSP10 gene functions in P. solenopsis. |
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