| ,2014,昆虫JHE基因结构及与甲基化相关的CpG O/E值分析[J].环境昆虫学报,(5):705-710 |
| 昆虫JHE基因结构及与甲基化相关的CpG O/E值分析 |
| Insect JHE gene structure and analysis of CpG O/E value related with methylation |
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| DOI： |
| 中文关键词: 保幼激素酯酶 DNA甲基化 基因结构 CpG O/E值 外显子 |
| 英文关键词:juvenile hormone esterase DNA methylation genetic structure CpG O/E value exons |
| 基金项目:国家自然科学基金资助项目(31171844); 广东省自然科学基金资助项目(S2011010001353) |
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| 中文摘要: |
| 本研究选择西方蜜蜂、黑腹果蝇、致倦库蚊和家蚕四种昆虫为例，以降解保幼激素的特异性酯酶（juvenile hormone esterase，JHE）为研究对象，首先运用NCBI的Spidey在线软件将各昆虫的JHE基因与其相应的基因组序列作比对，分别确定各昆虫JHE基因的上游2000bp的具体序列和外显子及内含子区域。发现西方蜜蜂JHE基因含有7个外显子和6个内含子，而其余三种昆虫的JHE基因均含有6个外显子和5个内含子。接着，分别统计基因各区域的CpG，G，C位点的占有量，并计算出CpG O/E值（CpG位点的实际值与期望值之间的比值），发现各区域CpG O/E平均值的大小依次为：基因上游2000bp区>内含子区>外显子区，基因上游2000bp区的CpG O/E平均值大于1.0，而第1到第4外显子的CpG O/E平均值均只有0.8左右。表明在昆虫JHE基因中，若有CpG甲基化位点发生，应主要发生在第1到第4外显子区域。 |
| 英文摘要: |
| This research selected 4 kinds of insects, Apis mellifera, Drosophila melanogaster, Culex quinquefasciatus and Bombyx mori to analyze the structures and CpG O/E value of juvenile hormone esterase (JHE) genes. First, the JHE genes were compared with their corresponding genomic sequences by using Spidey software online of NCBI to identify the 2000bp upstream sequence, exon and intron regions of these genes. The results showed that the JHE gene of Apis mellifera contained 7 exons and 6 introns, while the JHE genes of remaining three insects contained 6 exons and 5 introns. Then, analyzed the JHE gene regional share of CpG, G,C, CpG, and calculated CpG O/E values (the ratio between actual value and expected value of CpG locus ). The average values of CpG O/E in every area of JHE genes were found in the following order: gene upstream 2000bp > intron > exon, and the average value of CpG O/E in gene upstream area 2000bp was higher than 1, while the values in 1st-4th exon were 0.8 or so. This implied that if there were the CpG methylation sites in insect JHE genes, they should mainly happen in the 1st-4th exon regions. |
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