| 宋春满,邓建华,2012,云南烟蚜抗药性机制研究[J].环境昆虫学报,34(3):310-314 |
| 云南烟蚜抗药性机制研究 |
| The resistant mechanism of Myzus persicae to pesticide in Yunnan Province |
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| DOI：10.3969/j.issn.1674-0858.2012.03.07 |
| 中文关键词: 烟蚜 抗药性 解毒酶 靶标酶 机制 |
| 英文关键词:Myzus persicae insecticide-resistance detoxifcation enzyme target enzyme mechanism |
| 基金项目:云南省烟草公司科技计划项目（2011YN01） |
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| 中文摘要: |
| 通过比较云南烟蚜敏感品系和抗性品系的解毒酶（α-乙酸萘酯羧酸酯酶、β-乙酸萘酯羧酸酯酶）和靶标酶（乙酰胆碱酯酶）的活力，研究了烟蚜对有机磷、拟除虫菊酯和氨基甲酸酯类杀虫剂抗性的生化机制，并通过酯酶基因扩增检测和钠离子通道突变检测，研究了其抗性的分子机制。结果表明：α-乙酸萘酯羧酸酯酶活力增强是烟蚜对有机磷类、氨基甲酸酯类杀虫剂及拟除虫菊酯类杀虫剂的抗性机制之一；乙酰胆碱酯酶在烟蚜对有机磷杀虫剂抗性中起重要作用；3个抗性品系烟蚜均没有发生酯酶基因扩增，抗拟除虫菊酯品系烟蚜发生了钠离子通道突变。 |
| 英文摘要: |
| To study the biochemical and molecular mechanism of resistance to organophosphorus，pyrethroid and carbarmate pesticide，the activity of the detoxicating enzymes （α-NA carboxylesterase，β-NA carboxylesterase） and the target enzyme （acetylcholinesterase） were compared among the susceptible and the resistant strain of Myzus persicae. The results showed that enhanced α-NA carboxylesterase played an important role in the resistance to the three kinds of pesticides，so did the acetylcholinesterase to organophosphorus pesticide. All the three resistant strains had no amplified esterase genes，and the strain resistant to pyrethroid had a mutation in sodium channel. |
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