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| 转miR-34-5p玉米的构建及其对草地贪夜蛾幼虫的毒力评价 |
| Construction of miR-34-5p maize and its toxicity evaluation against Spodoptera frugiperda larvae |
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| DOI： |
| 中文关键词: 草地贪夜蛾 miR-34-5p 转基因玉米 RNA干扰 害虫防治 |
| 英文关键词:Spodoptera frugiperda miR-34-5p genetically modified maize RNA interference pest control |
| 基金项目:国家自然科学基金（32302347）；河南省农业科学院自主创新项目（2025ZC56）；河南省农业科学院优秀青年科技基金（2026YQ08）；2025年度河南省重大科技专项（251100110300）；博士科研启动费项目（2026BX44）；河南省农业科学院科技创新团队专项（2024TD18） |
| Author Name | Affiliation | | LI Lin-Hong, LI Tong, GONG Zhong-Jun, JIANG Yue-Li, DUAN Yun, WU Yu-Qing, LIANG Pei, MIAO Jin | 1. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450000
2. China Agricultural University, Beijing 100193 |
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| 中文摘要: |
| 【目的】草地贪夜蛾Spodoptera frugiperda是一种繁殖能力强、危害范围广的重要农业害虫，且已对多种杀虫剂产生了抗性，亟需开发新型绿色防控技术。【方法】前期研究发现，过表达或抑制鳞翅目昆虫的miR-34-5p，可显著提高草地贪夜蛾等多种鳞翅目害虫的死亡率，为进一步评价miR-34-5p在害虫防治中的应用潜力，本研究构建了表达miR-34-5p的转基因玉米，草地贪夜蛾取食该玉米96 h后，草地贪夜蛾体内miR-34-5p的表达量上调了2.5倍，其靶标基因蜕皮激素受体SfEcR的表达量显著降低了45.5%。【结果】生物测定结果表明，与取食野生型玉米相比，取食转miR-34-5p玉米的草地贪夜蛾累积死亡率显著升高了25.0%~40.0%。同时，存活幼虫的生长发育严重受阻，繁殖力显著降低。【结论】本研究证实了基于昆虫miRNA的转基因作物对草地贪夜蛾具有良好的防治效果，为开发基于miRNA的绿色害虫防治策略提供了理论基础和技术支撑。 |
| 英文摘要: |
| 【Aim】The fall armyworm, Spodoptera frugiperda, is a major agricultural pest with high reproductive potential and poses a significant threat to a wide range of crops. Currently, it has developed resistance to multiple insecticides. Therefore, alternative green control strategies are urgently needed.【Methods】Previous research revealed that overexpression or inhibition of microRNA, miR-34-5p, resulted in high mortality in multiple lepidopteran pests such as Spodoptera frugiperda. To further evaluate the potential application of miR-34-5p in pest control, we constructed genetically modified maize expressing miR-34-5p. After the fall armyworm fed on this maize for 96 hours, the expression level of miR-34-5p significantly increased by 2.5 fold, and the expression level of its target gene, the ecdysteroid receptor SfEcR, significantly decreased by 45.5%. 【Results】Bioassay results demonstrated that the cumulative mortality of S. frugiperda fed on miR-34-5p transgenic maize were significantly increased by 25.0%~40.0%, compared with that of larvae fed on wild-type maize. Surviving larvae exhibited significant developmental defects and low fecundity.【Conclusion】These results indicate that insect microRNA-based transgenic crops exhibit promising control efficacy against S. frugiperda, which provides a theoretical basis and technical support for the development of miRNA-based green pest control strategies. |
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