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| 基于线粒体COI基因的广西豇豆蓟马种群遗传分化分析 |
| Genetic differentiation analysis of cowpea thrips populations in Guangxi based on mitochondrial COI gene |
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| DOI： |
| 中文关键词: 豇豆蓟马 COI基因 种群鉴定 遗传分化 |
| 英文关键词:Cowpea thrips COI genes population identification genetic differentiation |
| 基金项目:广西重点研发计划（桂科农 AB2506910020）；广西自然科学基金（2023GXNSFAA026464）；国家重点研发计划（2024YFD1400105） |
| Author Name | Affiliation | | WANG Huan-Ting, HUANG Li-Fei, GONG Rui, CAO Xue-Mei, ZHENG Xia-Lin, YANG Lang | 1. College of Agriculture, Guangxi University, Nanning 530004, China
2. Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs/Plant Protection Research Institution, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
3. School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, China |
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| 中文摘要: |
| 【目的】为了明确广西田间豇豆蓟马的发生种类，【方法】采集其14个设区市虫源，利用线粒体COI基因测序并分析蓟马种群间的多样性、遗传分化和系统发育。【结果】本次合计扩增234条序列片段，识别到广西豇豆田间共5种蓟马，为普通大蓟马Megalurothrips usitatus、花蓟马Frankliniella intonsa、黄胸蓟马Thrips hawaiiensis、棕榈蓟马Thrips palmi和华简管蓟马Haplothrips chinensis，其中优势种为普通大蓟马。分析序列得出，普通大蓟马共计7个单倍型；花蓟马有6个单倍型；黄胸蓟马和华简管蓟马为5个单倍型；棕榈蓟马仅2个单倍型。普通大蓟马多数地区种群间存在遗传分化和基因交流，其次是花蓟马和华简管蓟马。除个别地区外，黄胸蓟马和棕榈蓟马的总体采样区域内分化程度较小。【结论】这一研究成果为当地豇豆蓟马制定科学合理的防治策略提供依据。 |
| 英文摘要: |
| 【Aim】To elucidate the thrips species composition infesting cowpea fields of Guangxi,【Methods】systematic thrips sampling was conducted in cowpea fields across 14 prefecture-level municipalities. Mitochondrial cytochrome oxidase subunit I (COI) gene sequencing was employed to assess diversity, genetic differentiation, and phylogenetic relationships among thrips population.【Results】From the 234 successfully amplified sequence fragments, five thrips species were identified: Megalurothrips usitatus, Frankliniella intonsa, Thrips hawaiiensis, Thrips palmi, and Haplothrips chinensis. Among them, M. usitatus was the dominant species. Sequence analysis revealed seven distinct haplotypes in M. usitatus, six in F. intonsa, five each in T. hawaiiensis and H. chinensis, and two in T. palmi. Genetic differentiation and gene flow were observed among M. usitatus populations across most regions, followed by F. intonsa and H. chinensis. In contrast, T. hawaiiensis and T. palmi exhibited minimal genetic differentiation among most sampled locations, except in isolated cases.【Conclusion】This research provides a foundation for developing scientifically sound control strategies against cowpea thrips in the region. |
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