《环境昆虫学报》

柑橘木虱Diaphorina citri Kuwayama是柑橘新梢期的重要害虫，也是柑橘黄龙病田间传播的最主要媒介昆虫，严重威胁着柑橘产业的健康发展。掌握害虫的种群遗传结构，对于制定有效的防控策略具有重要的参考价值。本研究以柑橘木虱的线粒体COI基因为分子标记，开展江西省柑橘木虱的种群遗传结构和多样性研究。在16个种群316头柑橘木虱供试样本COI基因序列中，共检测到8种单倍型（Hap_1~ Hap_8），其中Hap_1占总群体的92.7%，是所有种群中的优势单倍型，单倍型网络图呈现典型的星型结构，且Hap_1处于中心位置，推测Hap_1为所有供试群体的祖先单倍型。总群体单倍型多样性指数（Hd）为0.139，核苷酸多样性指数（Pi）为0.00022，表现出较低的遗传多样性。XYYS种群与多数种群间分化指数（Fst）大于0.15，呈现出相对较高的分化程度，而其他种群间分化程度低。中性检验及碱基错配分布分析结果表明江西省柑橘木虱种群经历过快速的种群扩张。本研究所得结果为系统了解江西省柑橘木虱种群遗传结构提供了基础数据。

英文摘要:

Diaphorina citri Kuwayama, an important pest of young citrus leaves, is the primary vector of the putative bacterial agent that causes Citrus huanglongbing, posing a serious threat to the healthy development of the citrus industry. Elucidation of the genetic architecture of this insect is therefore indispensable for the devising of targeted and effective management strategies. In the present study, the mitochondrial cytochrome c oxidase subunit I (COI) gene was employed as a molecular marker to analyse the population genetic structure and diversity of D. citri across Jiangxi Province, China. Eight haplotypes (designated as Hap_1 ~ Hap_8) were identified in all the 316 D. citri individuals from 16 distinct populations. Hap_1 was the dominant haplotype in all populations, accounting for 92.7% of the total samples. The observed pattern of the haplotype network manifested as a typical star-shaped structure, with Hap_1 occupying a central position, thereby strongly suggesting its ancestral maternal status for the surveyed populations . The overall haplotype diversity (Hd) was 0.131, and the nucleotide diversity index (Pi) was 0.00022, suggesting a low level of genetic diversity within the entire population. The differentiation index (Fst) of XYYS between other populations was found to be greater than 0.15, indicating a high level of differentiation level, and a low level of differentiation among the other populations. The neutrality test and mismatch distribution analyses indicate that D. citri populations in Jiangxi province have undergone a recent rapid dynamic expansion. The results of the study results provide fundamental data for a systematic understanding of the genetic structure of D. citri populations in Jiangxi province.

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