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| 为害新疆春小麦的黑角负泥虫形态鉴定与COⅠ基因序列分析 |
| Morphological identification and COⅠ gene sequence analysis of the cereal leaf beetle Oulema melanopus (Chrysomelidae: Criocerinae) infesting spring wheat in Xinjiang, China |
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| DOI： |
| 中文关键词: 新疆青河县 负泥虫 外部形态 生殖器 COⅠ基因序列分析 |
| 英文关键词:Qinghe county cereal leaf beetle external morphology genitalia COI gene sequence analysis |
| 基金项目:新疆青河县基层农技推广体系建设项目；北京市自然科学基金与北京市教育委员会科技计划重点项目（KZ 201810020026）；北京市自然科学基金（6182002）；国家自然科学基金项目（31272099）；中央引导地方科技发展资金项目（236Z2902G） |
| Author Name | Affiliation | | QIAHEBAN·Hapazi, ZHANG Peng-Fei, GUO Jia-Luo, DAI Li-Zhen, WANG Jin-Zhong, NAWUERZIBAI·Tuerxun, REN Zheng-Guang, WU Yan-Sheng | 1. Agriculture Promotion Center of Qinghe county, Aletai 836200, Xinjiang Uygur Autonomous Region, China
2. Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs, College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing 102206, China
3. Hebei Provincial Jujube Kernel Utilization Technology Innovation Center, College of Chemical Engineering and Biotechnology, Xingtai University, Xingtai 054000, Hebei Province, China |
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| 中文摘要: |
| 为了明确来自中国新疆青河县危害春小麦的一种负泥虫的种类，对其成虫外部形态和生殖器官结构进行了观察，并分析了其线粒体COⅠ基因序列和单倍型。结果显示，成虫体长4.98~5.61 mm，头部深蓝色，触角黑色11节，前胸背板橙红色。鞘翅长3.44~3.67 mm，每侧具10列由密而深刻点组成的条纹。腿节（除基部）、胫节（除端部）为橙红色，其余为黑色。雄性阳茎内的插入器（鞭毛）粗壮略短，长518.99 μm，宽86.29 μm，轻微弯曲。雌性受精囊导管在近端部硬化严重，长496.95 μm，盘曲折叠1圈。这些外部形态特征和生殖器结构与黑角负泥虫Oulema melanopus相符。线粒体COⅠ序列分析结果显示，与来自其他国家的11头黑角负泥虫COⅠ序列的遗传距离范围为0~0.091，系统发育表明与黑角负泥虫、O. mauroi和O.duftschmidi的亲缘关系最近，单倍型分析结果显示，本序列单独属于一个单倍型。 |
| 英文摘要: |
| In order to clarify the species of a cereal leaf beetle feed on spring wheat in Qinghe County, Xinjiang, China, the external morphology and internal reproductive organ structure of their adults were observed, and the mitochondrial COⅠ gene sequence and haplotype were also analyzed. The results showed that the body length of adult was 4.98~5.61 mm, with a dark blue head, 11-segmented black antennae, and an orange-red pronotum. Elytra were 3.44~3.67 mm in length, covered with 10 striae composed of dense and deep punctures. The femora (except base) and tibiae (except apex) were orange-red, while the remainder was black. The intromittent organ (flagellum) in male aedeagus is short, stout, and only slightly curved, with a length of 518.99 μm and a width of 86.29 μm. The ductus spermathecae is approximately 496.95 μm in length, with a heavily sclerotized proximal end, and coiled once. These external morphological features and genital structures are consistent with Oulema melanopus. Mitochondrial COI sequence analysis showed that the genetic distances compared to 11 other O. melanopus sequences from other countries ranged from 0~0.091, and the phylogenetic analysis also indicated that O. melanopus, O. mauroi, and O. duftschmidi were closest affinities. Haplotype analysis showed that this sequence belonged to one haplotype. |
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