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| 末黑露尾甲线粒体基因组全序列测定与扁甲总科系统发育分析 |
| Complete mitochondrial genome sequencing of Epuraea terminalis (Coleoptera: Nitidulidae) and phylogenetic analysis of Cucujoidea |
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| DOI： |
| 中文关键词: 末黑露尾甲 鞘翅目 露尾甲科 线粒体基因组 系统发育 |
| 英文关键词:Epuraea terminalis Coleoptera Nitidulidae mitochondrial genome phylogeny |
| 基金项目:山西省现代农业产业技术体系（2025CYJSTX03-29） |
| Author Name | Affiliation | | ZHANG Miao, KANG Chen, XING Kun, ZHAO Fei | 1. College of Plant Protection, Shanxi Agricultural University, Taiyuan 0300312, China
2. Shennong Technology Group Co., Ltd., Jinzhong 030801, Shanxi Province, China |
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| 中文摘要: |
| 本研究利用Illumina MiSeq测序平台对末黑露尾甲Epuraea terminalis线粒体基因测序后，进行基因组序列拼装、注释和特征分析。采用最大似然法和贝叶斯法构建了基于38种扁甲总科Cucujoidea昆虫线粒体的蛋白编码基因（PCGs）序列的系统发育树。末黑露尾甲线粒体基因组全序列大小为15 666 bp（GenBank 登录号：PP350830），具有22个tRNA基因，13个PCGs，2个rRNA基因和1段非编码控制区。在13个PCGs中，有11个的起始密码子为ATN，其余两个PCGs的起始密码子分别为TTG（nad1）和ACC（cox1）；有10个蛋白编码基因的终止密码子为TAA或T（AA），其余3个基因（atp8，cob，nad1）终止密码子为TAG。只有trnS1基因缺少DHU臂，其余21个tRNA基因均为三叶草结构。38种扁甲总科昆虫线粒体系统发育树结果显示，露尾甲科Nitidulidae、蜡斑甲科Helotidae及大蕈甲科Erotylidae聚在一个分支上。两种系统发育树的结果均显示末黑露尾甲与Epuraea guttata的亲缘关系最近。 |
| 英文摘要: |
| Using the Illumina MiSeq sequencing platform, we sequenced and analyzed the complete mitochondrial genome of Epuraea terminalis (Coleoptera: Nitidulidae). The mitochondrial genome was assembled, annotated, and characterized. Phylogenetic trees were constructed using both Maximum Likelihood (ML) and Bayesian methods based on the protein-coding gene (PCG) sequences of 38 species within the superfamily Cucujoidea. The complete mitochondrial genome of E. terminalis was 15 666 bp in length (GenBank accession number: PP350830), containing 13 PCGs, 22 tRNA genes, 2 rRNA genes, and a single non-coding control region. Among the 13 PCGs, 11 possessed the typical ATN start codon, while the remaining two used TTG (nad1) and ACC (cox1) as initiation codons, respectively. Ten PCGs terminated with the termination codon TAA or T(AA), whereas atp8, cob, and nad1 utilized TAG as stop codons. Only the trnS1 gene lacked the DHU arm, while all other 21 tRNA genes adopted the typical cloverleaf structure. The phylogenetic analysis based on the mitochondrial genomes of 38 Cucujoidea species revealed that the families Nitidulidae, Helotidae, and Erotylidae form a monophyletic clade. Both ML and Bayesian trees consistently indicate that E. terminalis is most closely related to Epuraea guttata. |
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