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| 高温低温催青下二化性家蚕蛹期Br-SG转录组分析 |
| Transcriptome analysis of Br-SG in pupal stage of Bombyx mori under high and low temperature incubation conditions |
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| DOI： |
| 中文关键词: 家蚕 滞育 蛹期 滞育激素 脑-咽下神经节 |
| 英文关键词:Silkworm diapause pupal stage diapause hormone Br-SG |
| 基金项目:国家重点研发计划（2023YFD1600901，2023YFF1103801）；国家自然科学基金重点项目（31830094，U20A2058）；国家现代农业产业技术体系资助（CARS-18-ZJ0102，CARS-18-ZJ0103）；重庆市自然科学基金创新群体项目（cstc2021jcyj-cxtt0005）；宜宾市蚕桑产业高效生产技术创新与集成示范（XNDX2022020015）；西南大学高层次人才项目（SWURC2021001） |
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| 中文摘要: |
| 滞育是昆虫为了躲避不利生存环境而进化出的一种发育停滞的适应性策略，家蚕Bombyx mori作为典型的胚胎滞育昆虫，其滞育诱导过程已得到广泛的研究，但蛹期滞育激素（DH）释放过程的分子机制仍不十分清楚。二化性家蚕滞育性受到母性跨世代影响，蚕卵在25℃催青时，蛹期DH经咽下神经节（SG）释放，产滞育卵；而蚕卵在15℃催青时，蛹期不释放DH，产非滞育卵。本研究分别对蚕卵采用25℃和15℃催青，在DH释放关键时期（蛹期第3天）解剖雌蛹脑-咽下神经节（Br-SG）进行转录组测序。共鉴定到888个差异表达基因，相比于25℃催青组，在15℃催青组中有568个上调表达基因和320个下调表达基因。GO分类和KEGG功能富集分析结果显示差异基因主要具有丝氨酸内肽酶活性、单加氧酶活性、GTP酶激活活性和G蛋白偶联受体活性；同时差异基因主要参与昆虫激素生物合成、萜类骨架生物合成、长寿调节途径-多物种、神经活性配体-受体相互作用等相关通路。该研究为进一步解析家蚕滞育诱导过程中蛹期DH释放分子机制提供靶标基因和参考。 |
| 英文摘要: |
| Diapause is an adaptive strategy characterized by the arrest of growth or reproductive development, which enables insects to survive from adverse living environment. The diapause induction process in the silkworm, a typical embryonic diapause insect, has been extensively studied. However, the molecular mechanisms governing the release of the diapause hormone (DH) during pupal stage remain unclear. The diapause characteristics of bivoltine silkworms are transgenerationally induced as a maternal effect. When eggs are incubated at 25°C, DH is released from the suboesophageal ganglion (SG) of female pupa, resulting in the production of diapause eggs by adult moths. In contrast, incubation at 15°C suppresses DH release, leading to the laying of non-diapause eggs. In this study, the eggs of silkworm were incubated at 25°C and 15°C respectively. During the critical period of DH release (day 3 of the pupal stage), brain-suboesophageal ganglion (Br-SG) complexes were dissected from female pupae for transcriptome sequencing. Comparative analysis revealed 888 differentially expressed genes (DEGs). Among these, 568 genes were up-regulated and 320 genes were down-regulated in the 15°C incubation group relative to the 25°C incubation group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that these DEGs were predominantly associated with molecular functions such as serine-type endopeptidase activity, monooxygenase activity, GTPase activation activity and G protein-coupled receptor activity. Additionally, the DEGs were found to be primarily enriched in pathways related to hormone biosynthesis, terpenoid skeleton biosynthesis, longevity regulation pathway-multi-species, neural activity ligand-receptor interaction and other related pathways. This study identifies key target genes and establishes a foundational framework for elucidating the molecular mechanism underlying DH release during the pupal stage in the diapause induction process of silkworm. |
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