|
| 云南草地贪夜蛾田间种群对Bt毒素的抗性基因频率监测研究 |
| Monitoring research on the resistance gene frequency of field populations of Spodoptera frugiperda to Bt toxin in Yunnan |
| |
| DOI： |
| 中文关键词: 草地贪夜蛾 苏云金芽孢杆菌 Cry1Ab Vip3Aa 抗性基因频率 |
| 英文关键词:Spodoptera frugiperda Bacillus thuringiensis Cry1Ab Vip3Aa resistance gene frequency |
| 基金项目:科技创新2030重大项目（2022ZD0402104）；中国农业科学院青年创新专项（Y2025QC37）；中国农业科学院科技创新工程科学中心重点任务（CAAS-CSCB-202303） |
| Author Name | Affiliation | | KANG Qiang-Qiang, JIN Ming-Hui, YANG Xian-Ming, WU Kong-Ming, LI Yi-Ping, XIAO Yu-Tao | 1. Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
2. Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
3. State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
|
| Hits: 868 |
| Download times: 381 |
| 中文摘要: |
| 转基因Bt作物的广泛应用和产业化推广不仅有效遏制了虫害的大规模暴发，显著减少了化学农药的施用量，更重要的是缓解了由此带来的农药残留及环境污染问题。为了监测我国云南地区草地贪夜蛾Spodoptera frugiperda田间种群对Bt毒素的抗性，本研究以云南寻甸地区草地贪夜蛾田间种群为研究对象，通过F2代结合诊断剂量法对草地贪夜蛾田间种群进行抗性监测，系统评估了该地区草地贪夜蛾对Bt毒素的抗性水平。检测结果显示，仅发现3个单雌系对Cry1Ab具有低频抗性（抗性等位基因频率为0.006），而对Vip3Aa仍保持高度敏感。本研究表明云南地区草地贪夜蛾种群尚未形成明显抗性，这为该区域Bt转基因作物的可持续推广提供了重要科学依据，并建议继续开展长期监测和抗性治理策略研究。 |
| 英文摘要: |
| The wide application and industrialization promotion of transgenic Bt crops not only effectively contained the large-scale outbreak of pests, significantly reduced the application amount of chemical pesticides, but more importantly, alleviated the resulting problems of pesticide residues and environmental pollution. In order to monitor the resistance of S. frugiperda to Bt toxin in Yunnan Province, this study investigated the field populations of fall armyworm in Xundian County, Yunnan Province; diagnostic dose test in the F2 generation was performed. The research systematically assessed the level of resistance of fall armyworm populations in this area to Bt toxins. Detection results showed that only three single female lines had low-frequency resistance to Cry1Ab (resistance allele frequency of 0.006), but remained highly sensitive to Vip3Aa. This study indicated that the fall armyworm population in Yunnan has not yet developed obvious resistance, providing an important scientific basis for the sustainable promotion of Bt transgenic crops in this area and suggesting the need for long-term resistance monitoring and resistance management strategies. |
| View Full Text View/Add Comment Download reader |
| Close |
|
|
|
About Journal