|
| 基于比较转录组测序的亚洲玉米螟幼虫滞育相关基因筛选 |
| Screening of genes associated with diapause in Ostrinia furnacalis larvae based on comparative transcriptome sequencing |
| |
| DOI： |
| 中文关键词: 亚洲玉米螟 幼虫 转录组 滞育关联基因 生物信息学 |
| 英文关键词:Ostrinia furnacalis larvae transcriptome diapause associated gene bioinformatics |
| 基金项目: |
|
| Hits: 12 |
| Download times: 6 |
| 中文摘要: |
| 为明确亚洲玉米螟Ostrinia furnacalis幼虫滞育的分子机制，挖掘潜在的滞育关联基因和信号通路，利用Illumina NovaSeqTM 6000高通量测序平台对亚洲玉米螟非滞育（Non-diapause，ND）、滞育（Diapause，D）及解除滞育（Diapause-termination，DT）的幼虫进行测序，并进行生物信息学分析和滞育相关基因的筛选。利用qRT-PCR技术对转录组测序结果进行验证。结果显示D vs ND和DT vs D比较组分别获得3 520和1 107个差异表达基因。D vs ND组差异表达基因功能主要富集到蛋白酶体、氨基酸和核苷酸糖代谢、内质网中的蛋白质加工和酪氨酸代谢通路；DT vs D组差异表达基因功能主要富集于缬氨酸、亮氨酸和异亮氨酸生物合成、氧化磷酸化、甘氨酸、丝氨酸和苏氨酸代谢、其他聚糖降解和氮代谢通路。随机选取9个差异表达基因的qPCR验证结果与转录组测序结果一致。进一步挖掘出调控滞育的蜕皮激素相关基因7个、保幼激素相关基因20个、生物钟信号通路相关基因8个、Ca2＋信号通路相关基因21个和胰岛素相关基因5个，推测这些基因在亚洲玉米螟幼虫滞育调控中起着重要作用，为筛选亚洲玉米螟绿色防控分子靶标提供必要的基础。 |
| 英文摘要: |
| To elucidate the molecular mechanisms underlying diapause in the larvae of Ostrinia furnacalis, we aimed to identify potential diapause-associated genes and signaling pathways. We employed the Illumina NovaSeqTM 6000 high-throughput sequencing platform to sequence the O. furnacalis larvae in three states: non-diapause (ND), diapause (D), and diapause-termination (DT). Subsequent bioinformatics analyses were conducted to screen for diapause-related genes. The transcriptomic sequencing results were validated using qRT-PCR. Comparisons between D vs ND and DT vs D yielded 3,520 and 1,107 differentially expressed genes (DEGs), respectively. Functional enrichment analysis of DEGs in the D vs ND group primarily highlighted pathways related to the proteasome, amino acid and nucleotide sugar metabolism, protein processing in the endoplasmic reticulum, and tyrosine metabolism. In contrast, the DT vs D group showed significant enrichment in pathways associated with the biosynthesis of valine, leucine, and isoleucine; oxidative phosphorylation; glycine, serine, and threonine metabolism; and other polysaccharide degradation and nitrogen metabolism pathways. qPCR validation of nine randomly selected DEGs corroborated the transcriptomic sequencing results. Furthermore, we identified seven ecdysteroid-related genes, twenty juvenile hormone-related genes, eight circadian rhythm signaling pathway-related genes, twenty-one Ca2+ signaling pathway-related genes, and five insulin-related genes that are hypothesized to play crucial roles in the regulation of diapause in O. furnacalis larvae. This study provides essential groundwork for the identification of molecular targets for the eco-friendly control of O. furnacalis. |
| View Full Text View/Add Comment Download reader |
| Close |
|
|
|
About Journal