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| 橘小实蝇唾液腺细菌鉴定及其生物活性研究 |
| Identification of bacteria in the salivary gland of Bactrocera dorsalis (Hendel) and study of their bioactivity |
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| DOI： |
| 中文关键词: 橘小实蝇 共生菌 引诱作用 肺炎克雷伯氏菌 2-苯乙醇 |
| 英文关键词:Bactrocera dorsalis (Hendel) symbiotic bacteria attractiveness Klebsiella pneumoniae phenethyl alcohol |
| 基金项目:国家重点研发计划（2023YFD1401400） |
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| 中文摘要: |
| 昆虫消化道微生物群落可显著调控宿主行为趋性，唾液腺作为消化道上游的特殊微生境，其菌群对趋性行为的潜在作用亟待探究。本研究以橘小实蝇Bactrocera dorsalis为对象，聚焦其唾液腺微生物群落，筛选和鉴定了对成虫具有引诱趋性效果的功能菌株及关键化合物。成功分离鉴定布甘多肠杆菌Enterobacter bugandensis、铜绿假单胞菌Pseudomonas aeruginosa、肺炎克雷伯氏菌Klebsiella pneumoniae和法氏柠檬酸杆菌Citrobacter farmeri。行为学实验表明：室内条件下肺炎克雷伯氏菌与法氏柠檬酸杆菌对成虫均具显著引诱效果；而野外环境中仅肺炎克雷伯氏菌维持高效诱集能力。通过气相色谱-质谱联用（GC-MS）与触角电位（EAD）技术联用，证实肺炎克雷伯氏菌培养上清液中2-苯乙醇可特异性激发橘小实蝇触角电生理响应。室内引诱试验进一步显示，0.05 μL/mL 2-苯乙醇溶液对成虫引诱效果显著。本研究首次揭示唾液腺内肺炎克雷伯氏菌可能通过代谢产物2-苯乙醇来调控橘小实蝇趋性行为，同时肺炎克雷伯氏菌发酵液在野外环境具有显著的引诱效果，为开发基于该微生物菌剂的橘小实蝇绿色监测与防控技术提供了新策略和重要理论依据。 |
| 英文摘要: |
| The microbial community within the insect digestive tract can exert a substantial regulatory influence on the behavioral tendencies of the host. The salivary glands, serving as a unique microenvironment upstream of the digestive tract, are potentially implicated in the behavior of attraction. Focusing on the microbial community in the salivary glands of the oriental fruit fly, Bactrocera dorsalis, this study have screened and identified functional bacteria strains and key compounds that exhibit an attracting effect on adult flies. Enterobacter bugandensis, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Citrobacter farmeri were isolated and characterized. Behavioral assays demonstrated that both K. pneumoniae and C. farmeri had significant attracting effects on adult flies under indoor conditions. In the wild environment, however, only K. pneumoniae retained a high efficiency in attracting adult flies. By integrating gas chromatography-mass spectrometry (GC-MS) and electroantennogram (EAG) techniques, it was confirmed that phenethyl alcohol in the culture supernatant of K. pneumoniae could specifically elicit an electrophysiological response from the antennae of B. dorsalis. Further indoor attraction experiments revealed that a 0.05 μL/mL phenethyl alcohol solution had a notable attracting effect on adult flies. This study was the first to disclose that K. pneumoniae in the salivary glands of the oriental fruit fly might modulate the fly's behavioral tendency via the metabolite phenethyl alcohol. Simultaneously, the fermentation broth of K. pneumoniae exhibits a remarkable attracting effect in the wild environment. This finding offers a novel strategy and crucial theoretical foundation for the development of green monitoring and control technologies for B. dorsalis. |
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