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| 基于表皮碳氢化合物的六种鳞翅目成虫鉴定技术 |
| Identification of six species of lepidopteran adults by using cuticular hydrocarbons |
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| DOI： |
| 中文关键词: 表皮碳氢化合物 鳞翅目 成虫 气相色谱-质谱联用仪 物种鉴定 |
| 英文关键词:Cuticular hydrocarbons Lepidoptera adults GC-MS taxonomic identification |
| 基金项目:广西自然科学基金粤桂联合基金（2022GXNSFDA080003）；海南省自然科学基金（322MS093）；广东省现代农业产业技术体系创新团队建设项目（2024CXTD21） |
| Author Name | Affiliation | | SHI Qing-Xing, CHEN Ting, CHEN Jie, GUAN Zi-Ying, LU Jia-Jin, HU Gao, QI Guo-Jun | 1. Guangdong Provincial Key Laboratory of High Technology for Plant Protection/Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
2. Sanya Institute of Nanjing Agricultural University, Sanya 572024, Hainan Province, China
3. National Hainan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, Hainan Province, China |
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| 中文摘要: |
| 基于表皮碳氢化合物的化学分类法已成为昆虫分类鉴定的重要补充工具。本研究以草地贪夜蛾Spodoptera frugiperda（J.E.Smith）、甜菜夜蛾S. litura（Fabricius）、斜纹夜蛾S. exigua（Hübner）、东方黏虫Mythimna separata（Walker）、棉铃虫Helicoverpa armigera（Hübner）和亚洲玉米螟Ostrinia furnacalis（Guenée）为对象，利用热脱附固体进样法和气相色谱-质谱联用仪分析6种常见鳞翅目害虫雌雄成虫的表皮碳氢化合物种类和相对含量，并进行主成分分析、判别分析及聚类分析。结果表明：6种鳞翅目害虫共检测到79种表皮碳氢化合物，包括直链烷烃11种、单甲基烷烃42种、二甲基烷烃17种和未知碳氢化合物9种；判别分析可准确区分6种鳞翅目成虫，且能有效区分雌雄个体；聚类分析结果表明，在亚科水平上的聚类结果与传统形态学及分子鉴定的亲缘关系一致，但在亚科以下水平的种间聚类结果存在差异。因此，表皮碳氢化合物组成分析为鳞翅目成虫种类鉴定提供了有效手段，尤其适用于残损样本鉴定，在虫情测报及口岸入侵物种监测中具有应用潜力。 |
| 英文摘要: |
| Chemotaxonomy based on cuticular hydrocarbons (CHCs) serves as a critical supplementary tool for insect taxonomic identification. The composition and relative abundance of CHCs in male and female adults of six common lepidopteran species, including Spodoptera frugiperda (J.E.Smith), S. litura (Fabricius), S. exigua (Hübner), Mythimna separata (Walker), Helicoverpa armigera (Hübner), and Ostrinia furnacalis (Guenée), were analyzed by Thermal Separation Probe (TSP) and Gas Chromatography-Mass Spectrometry (GC-MS). Principal component analysis (PCA), discriminant analysis (DA), and cluster analysis (CA) were applied to the CHC profiles. The results revealed that a total of 79 distinct CHCs were detected across the six lepidopteran species, including 11 n-alkanes, 42 monomethyl alkanes, 17 dimethyl alkanes, and 9 unidentified hydrocarbons. Discriminant analysis accurately distinguished all six lepidopteran species and highlighted significant sexual dimorphism in their CHC profiles. Cluster analysis results showed agreement with morphological and molecular phylogenetic relationships at the subfamily level, but exhibited incomplete consistency in interspecific clustering relationships below their taxonomic level. These findings demonstrate that CHC composition provides a robust method for species-specific identification of lepidopteran adults, particularly for damaged specimens, with potential applications in pest monitoring and forecasting, as well as rapid quarantine screening of invasive alien species at ports. |
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