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| 食叶幼虫肠道中细菌群落结构的影响因素及功能冗余分析 |
| Analysis of influencing factors and functional redundancy of bacterial community structure in the gut of folivorous larvae |
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| DOI： |
| 中文关键词: 食叶幼虫 16S rRNA 细菌群落结构 功能冗余 |
| 英文关键词:Folivorous larvae 16S rRNA bacterial community structure functional redundancy |
| 基金项目:国家自然基金面上项目（32170421）；北京市自然基金面上项目（5232001）；“十四五”北京市属高校高水平科研创新团队支持计划项目（BPHR20220114） |
| Author Name | Affiliation | | ZHENG Yu-Xuan, YANG Cai-Qing, WANG Ying, WANG Gui-Fang, DAI Shan-Shan, ZHANG Ai-Bing | 1. College of Life Science, Capital Normal University, Beijing 100048, China
2. China National Environmental Monitoring Centre, Beijing 100012, China |
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| 中文摘要: |
| 昆虫肠道细菌群落在宿主营养代谢、解毒及适应性进化中具有重要作用，但其群落结构形成与维持的驱动机制及功能关联尚需系统解析。本研究以“食叶幼虫-植物-细菌群落”为研究系统，探讨影响食叶幼虫肠道细菌群落结构的因素及细菌群落结构与功能关系。野外采集跨越3个不同的地理区域（北京、浙江和四川），获得304头食叶幼虫、142份寄主植物及106份土壤样本。利用DNA条形码技术和形态学鉴定方法确定昆虫与植物物种，结合16S rRNA扩增子测序（V3-V4区）探究宿主分类特异性、寄主植物、环境微生物（叶际与土壤）对肠道菌群的影响，并基于PICRUSt2功能预测方法评估食叶幼虫肠道菌群的功能冗余。结果表明，食叶幼虫物种身份与寄主植物物种身份对食叶幼虫肠道细菌群落结构的影响随分类阶元细化（目→种）而增加并在物种水平达到最高。其中幼虫物种水平的分类因素解释了肠道细菌群落结构变异的30.27%（R2 = 0.3027，F = 3.2457，P < 0.001），寄主植物在物种水平的分类因素解释了肠道细菌群落变异的28.73%（R2 = 0.2873，F = 4.1882，P < 0.001）。叶际微生物群与土壤微生物群及采样点差异对昆虫肠道细菌群落结构均具有显著影响（P < 0.001），但影响强度均小于昆虫物种身份及昆虫取食差异。在物种水平上，65.37%~70.56%的食叶幼虫物种对之间表现出肠道细菌群落结构的显著差异，但仅有25.29%~26.71%的物种对之间表现出细菌群落功能上的差异，表明肠道细菌功能冗余在食叶幼虫肠道中普遍存在。 |
| 英文摘要: |
| Insect gut bacterial communities play an important role in host nutrient metabolism, detoxification and adaptive evolution. However, the driving mechanisms and functional correlations that shape and maintain the community structure still require systematic analysis. This study established a "folivorous larvae - plants - bacteria community" tripartite system to investigate the factors influencing gut bacterial community structure of folivorous larvae and structure-function relationships within these bacterial communities. Field collections spanned three distinct geographic regions (Beijing, Zhejiang, and Sichuan), yielding 304 larval specimens, 142 host plant samples, and 106 soil samples. DNA barcoding and morphological identification were utilized to determine species for both insects and plants. Through 16S rRNA amplicon sequencing (V3-V4 regions), we assessed how host taxonomy, host plants, and environmental microorganisms (phyllosphere/soil) shape insect gut microbiota. PICRUSt2 functional predictions were employed to test for functional redundancy in larval gut microbiota. The results showed that the effects of folivorous larvae species identity and host plants identity on gut microbiota structure intensified with taxonomic resolution (Order→Species), peaking at the species level. The taxonomic factors at the larval species level explained 30.27% of the structural variations of the gut bacterial community (R2 = 0.3027, F = 3.2457, P < 0.001) and the taxonomic factors of host plants at the species level explained 28.73% of the variations (R2 = 0.2873, F = 4.1882, P < 0.001), respectively. While phyllosphere microbiota, soil microbiota, and sampling sites significantly influenced gut community structure（P < 0.001）, their effects were subordinate to larval species identity and dietary differences. At the species level, significant divergence in gut bacteria composition was detected across 65.37%~70.56% of folivorous larvae species pairs. But only 25.29%~26.71% of these pairs exhibited functionally distinct microbial profiles, demonstrating widespread functional redundancy within gut bacterial communities among these folivorous larvae. |
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