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| 转录因子NlMyc在调控褐飞虱致害高麦黄酮抗性水稻的功能研究 |
| Functional study of transcription factor NlMyc in regulating the Virulence of the brown planthopper to resistance rice with high tricin Varieties |
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| DOI： |
| 中文关键词: 褐飞虱 麦黄酮 NlMyc RNAi 致害性 |
| 英文关键词:Nilaparvata lugens tricin NlMyc RNAi virulence |
| 基金项目: |
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| 中文摘要: |
| NlMyc是生物体中极其重要的转录因子，在多种细胞活动过程中起着至关重要的作用，包括细胞周期、细胞生长、增殖、凋亡等细胞过程。但目前转录因子如何调节褐飞虱Nilaparvata lugens（St?l）致害水稻的功能方面尚未报道。本研究克隆和鉴定了褐飞虱转录因子NlMyc基因，结果表明NlMyc基因全长1 830 bp，编码609个氨基酸。利用RT-qPCR技术检测NlMyc基因的抗性表达水平以及在褐飞虱体内的时空表达方式，研究发现褐飞虱取食抗性水稻后，NlMyc基因表达量显著高于取食感虫水稻时，且NlMyc基因在褐飞虱雌成虫1天和唾液腺组织中表达量最高。对NlMyc基因进行RNAi后，发现褐飞虱在高麦黄酮抗性水稻上的取食孔的数量为28.9 ± 8.5个，相比于对照组（Control、dsGFP）的13.5 ± 4.3个和16.2 ± 8.4个，取食孔数量显著增多；dsNlMyc、Control和dsGFP处理48 h的褐飞虱平均蜜露分泌量分别为2.44 mg、5.3 mg和4.6 mg；获得性体重为0.23 mg、0.49 mg和0.47 mg，dsNlMyc组相对于对照组的蜜露分泌量和获得性体重都表现出显著的下降，这说明沉默NlMyc基因降低了褐飞虱在抗性水稻上的适合度及取食能力，调控褐飞虱致害抗性水稻。本研究证明了NlMyc是调控褐飞虱致害高麦黄酮抗性水稻的关键转录因子，不仅为探索褐飞虱致害性变异提供了新的思路，而且为害虫防治提供了新的分子靶标。 |
| 英文摘要: |
| NlMyc is an extremely important transcription factor in living organisms and playing a crucial role in a various of cellular processes, including cell cycle, growth, proliferation, apoptosis and other cellular processes. However, it has not been reported how transcription factors regulate the function of brown planthopper (Nilaparvata lugens (St?l)) damage to rice. This study cloned and identified the transcription factor NlMyc gene of the brown planthopper. The results showed that the NlMyc gene has a total length of 1 830bp and encodes 609 amino acids. Using RT-qPCR technology to detect the resistance expression level of NlMyc gene and its spatio-temporal expression pattern in brown planthopper, the study found that after brown planthopper fed on resistant rice, the expression level of NlMyc gene was significantly higher than that of brown planthopper fed on susceptible rice, and the expression level of NlMyc gene was the highest in the 1-day and salivary gland tissues of brown planthopper. After RNAi of NlMyc gene, it was found that the number of feeding holes of brown planthopper on high tricin resistant rice was 28.9 ± 8.5, which was significantly increased compared to the Control group (Control, dsGFP) with 13.5 ± 4.3 and 16.2 ± 8.4 feeding holes; The average honeydew secretion of brown planthoppers treated with dsNlMyc, Control and dsGFP for 48 hours was 2.44 mg, 5.3 mg and 4.6 mg; The weight gain was 0.23 mg, 0.49 mg, and 0.47 mg, and the dsNlMyc group showed a significant decrease in honeydew secretion and weight gain compared to the Control group. This indicates that silencing the NlMyc gene reduced the fitness and feeding ability of brown planthoppers, and regulated the damage caused by brown planthopper on resistane rice. This study demonstrates that NlMyc is a key transcription factor regulating the damage caused by brown planthopper to high tricin resistant rice, which not only provides new ideas to explore the harmful variation of brown planthopper, but also provides new ideas molecular target for pest Control. |
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