《环境昆虫学报》

为探究草地贪夜蛾Spodoptera frugiperda在转录水平对不同寄主植物的适应机制，本研究对取食玉米和高粱的草地贪夜蛾幼虫进行转录组测序、功能注释与分类、差异表达基因的分析与验证。转录组结果显示，与取食玉米的草地贪夜蛾幼虫相比，取食高粱的草地贪夜蛾幼虫发现1 454个差异表达基因，其中上调表达基因860个，下调表达基因594个。GO功能和KEGG通路富集分析发现取食高粱的幼虫差异表达基因在碳水化合物代谢和毒物代谢等过程均显著富集，其中鉴定到39个差异表达解毒酶基因，包括9个UDP-葡萄糖醛酸转移酶、8个谷胱甘肽硫转移酶、3个羧基/胆碱酯酶、16个细胞色素P450和3个ATP结合盒转运蛋白基因。qRT-PCR验证进一步证实取食高粱极显著诱导了草地贪夜蛾幼虫UGT2-4、UGT33B13、GSTS1、CYP4G74和CYP4G15-2的表达。总之，本研究通过组学分析与分子验证表明这些上调的解毒酶基因在草地贪夜蛾幼虫对寄主高粱的适应中发挥了重要作用。

英文摘要:

To investigate the adaptation mechanism of the fall armyworm Spodoptera frugiperda to different host plants at the transcriptional level, this study carried out transcriptome sequencing, functional annotation and classification, as well as the analysis and verification of differentially expressed genes (DEGs) in larvae feeding on maize and sorghum. Transcriptomic analysis identified 1 454 DEGs in larvae feeding on sorghum relative to maize, including 860 upregulated genes and 594 downregulated genes. GO functional and KEGG pathway enrichment analyses showed that these DEGs were simultaneously enriched in processes related to carbohydrate and toxin metabolism, and so on. Among the DEGs, 39 DEGs were identified as detoxification enzyme genes, including 9 UDP-glucuronosyltransferase, 8 glutathione S-transferase, 3 carboxyl/cholinesterase, 16 cytochrome P450, and 3 ATP-binding cassette transporter genes. qRT-PCR validation further confirmed that sorghum feeding dramatically induced the expression of detoxification enzyme genes UGT2-4, UGT33B13, GSTS1, CYP4G74, and CYP4G15-2 in S. frugiperda larvae. Collectively, this study, supported by both transcriptomic analysis and molecular validation, suggests that these upregulated detoxification enzyme genes play an essential role in the adaptation of fall armyworm larvae to sorghum as a host.

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