《环境昆虫学报》

为探究美国白蛾Hyphantria cunea（Drury）地理种群系统发育关系以及黑头型和红头型的分化。本文对采自我国河北、河南、辽宁、山东、天津9个样点以及美国佐治亚州、伊利诺伊州、印第安纳州、堪萨斯州、肯塔基州、密歇根州、密苏里州、北卡罗来纳州、俄亥俄州、田纳西州、弗吉尼亚州17个样点的美国白蛾幼虫样本进行线粒体mt-COI基因扩增、测序和分析。结合GenBank下载的24条mt-COI序列，利用邻接法（Neighbor-joining，NJ）和最大似然法（Maximum likelihood，ML）重建美国白蛾系统发育关系，利用DnsSP6进行单倍型分析。结果表明：美国白蛾48条mt-COI序列聚成两个分支，代表了两种不同的幼虫头型，黑头型mt-COI基因序列进一步分成两支，第一个分支由中国、加拿大东海岸、美国中东部和东北部、日本茨城的地理种群聚成；第二个分支由美国南部和西部、日本名古屋的地理种群聚成。红头型和黑头型幼虫呈现出明显的种群分化。在黑头型美国白蛾中，中国和日本茨城的地理种群与加拿大东海岸和美国东北部的种群更接近，日本名古屋的地理种群与美国西部和南部的种群更接近，且日本种群较中国种群呈现出更明显的分化。

英文摘要:

To explore the phylogenetic relationships among geographical populations of Hyphantria cunea (Drury), and the divergence between black- and red-headed types. The complete mitochondrial genome of larvae samples collected from 9 localities in China (Liaoning, Hebei, Shandong, Henan Prov. and Tianjin) and 17 localities in the United States (Georgia, Illinois, Indiana, Kansas, Kentucky, Michigan, Missouri, North Carolina, Ohio, Tennessee and Virginia) were sequenced and analyzed. Combined with the 24 mt-COI sequences downloaded from GenBank, the phylogenetic relationships of H. cunea were reconstructed by using neighbor-joining and maximum likelihood methods, and the haplotype was analyzed by DnsSP6. The results showed that 48 mt-COI sequences clustered into two branches, representing two distinct larval head types. The blackhead type further clustered into two sub-branches, the first one is composed of the geographical species of China, the eastern coast of Canada, the mid-eastern and northeastern United States and Ibaraki, Japan; The second one consists of geographic colonies of the southern and western United States and Nagoya, Japan. The red- and black-headed morphs have significant population differentiation. Among balck-headed webworms, populations of China and Ibaraki, Japan were genetically closer to the eastern coast of Canada and the northeastern United States. Populations of Nagoya, Japan were closer to the western and southern United States, and Japanese fall webworm populations exhibited greater genetic differentiation than those in China.

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