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| 氯虫苯甲酰胺亚致死剂量对螟黄赤眼蜂寄生功能及主要解毒酶活性的影响 |
| Effects of sublethal dosage of chlorantraniliprole on function of parasitism and detoxifying enzymes activity in Trichogramma chilonis Ishii |
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| DOI： |
| 中文关键词: 氯虫苯甲酰胺 螟黄赤眼蜂 亚致死剂量 寄生 解毒酶 |
| 英文关键词:Chlorantraniliprole Trichogramma chilonis sublethal dosage parasitism detoxifying enzyme |
| 基金项目:国家现代农业（梨）产业技术体系资助项目(CARS-28)；山西省青年科学研究项目（202103021223133，20210302124024）；山西农业大学博士科研启动项目（2021BQ57）；山西省自然科学研究面上项目（202303021211089） |
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| 中文摘要: |
| 为了明确氯虫苯甲酰胺亚致死剂量对螟黄赤眼蜂Trichogramma chilonis Ishii寄生功能及主要解毒酶活性的影响，在实验室条件下观察氯虫苯甲酰胺处理后的螟黄赤眼蜂生长发育和寄生状况，并使用酶联免疫吸附试验（ELISA）测定了解毒酶活性。结果表明经氯虫苯甲酰胺处理后，螟黄赤眼蜂子代的羽化率从91.59%降至84.49%，发育历期由11.19 d缩短至9.92 d。螟黄赤眼蜂子代寄生卵数和寄生率均有不同程度的降低，其中子代寄生率在26.67%~38.00%。细胞色素P450酶活性和谷胱甘肽S-转移酶的酶活性均显著高于对照，其中细胞色素P450酶活性增加了19.23%，谷胱甘肽S-转移酶活性增加了33.75%。氯虫苯甲酰胺对细胞色素P450和谷胱甘肽转移酶有诱导作用，这两种解毒酶可能参与螟黄赤眼蜂对氯虫苯甲酰胺的代谢解毒。 |
| 英文摘要: |
| In order to clarify the effects of sublethal dosage of chlorantraniliprole on function of parasitism and detoxifying enzyme activity in Trichogramma chilonis, the growth development and parasitism of T. chilonis treated with sublethal dosage of chlorantraniliprole were observed and the detoxifying enzymes activity were determined by using ELISA method in the laboratory. The results showed that the emergence rate of offspring decreased from 91.59% to 84.49% and the developmental duration was shortened from 11.19 to 9.92 days. The number of parasitized eggs and parasitism rate decreased to vary degrees, with the parasitism rates were ranging from 26.67% to 38.00%. The activity of P450 and GST in treated T. chilonis were significantly higher than control, with an increase of 19.23% and 33.75%, respectively. Chlorantraniliprole had an inducing effect on P450 and GST, which may be involved in the metabolic detoxification of chlorantraniliprole in T. chilonis. |
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