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| miR-120对台湾乳白蚁抵御绿僵菌的免疫反应的影响 |
| Impact of miR-120 on the immune response of Coptotermes formosanus against Metarhizium anisopliae |
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| DOI： |
| 中文关键词: 台湾乳白蚁 miR-120 免疫反应 绿僵菌 白蚁防治 |
| 英文关键词:Coptotermes formosanus miR-120 immune response Metarhizium anisopliae termite control |
| 基金项目:广州市科技重点项目（201904020002）；广州市科技项目（2023A04J0147） |
| Author Name | Affiliation | | ZHANG Hong, LI Zhi-Qiang, JIA Feng-Long, CHEN Wei-Wen | 1. College of Life Science, Sun Yat-sen University, Guangzhou 510275, China
2. Institute of Zoology, Guangdong Academy of Sciences, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangzhou 510260, China |
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| 中文摘要: |
| 本研究探究了miR-120在台湾乳白蚁Coptotermes formosanus抵御绿僵菌Metarhizium anisopliae的免疫反应中的作用。研究发现通过对台湾乳白蚁注射miR-120 agomir以增加白蚁体内miR-120的含量后，台湾乳白蚁的存活率降低约35%，绿僵菌对台湾乳白蚁的致死率提升约55%，同时显著抑制了台湾乳白蚁对绿僵菌的趋避反应。双荧光素酶报告系统等试验证实了miR-120靶向结合免疫基因Draper。通过对Draper进行注射法RNAi后，得到了类似于注射miR-120 agomir后的结果。研究结果表明，miR-120通过抑制靶基因Draper的表达，抑制了台湾乳白蚁抵御绿僵菌的免疫反应，最终导致台湾乳白蚁的存活率显著下降。该结果有助于进一步理解白蚁的免疫机制，并为开发抑制白蚁免疫防御反应的技术方法提供理论基础，有助于促进白蚁生物防治的发展。 |
| 英文摘要: |
| This study investigated the role of miR-120 in the immune response of Coptotermes formosanus against Metarhizium anisopliae through a series of experiments. First, injecting C. formosanus with miR-120 agomir to increase the level of miR-120 in C. formosanus decreased the survival rate of C. formosanus by about 35% and increased the mortality rate of C. formosanus treated with M. anisopliae to about 55%. At the same time, the repellent response of C. formosanus to M. anisopliae was significantly inhibited. Following, some experiments including the dual luciferase reporter system were performed to confirm that miR-120 targets the immune gene Draper. Finally, by injecting RNAi to knockdown of Draper and obtained results similar to those obtained after injection of miR-120 agomir. These results suggested that miR-120 inhibits the immune response of C. formosanus to M. anisopliae by inhibiting the expression of the target gene Draper, which ultimately leaded to a significant decrease in the survival rate of C. formosanus. This study will help us to better understand the immune mechanism of termites and provide a theoretical basis for the development of technical methods to inhibit termite immune responses, which will help to promote the development of biological control of termites. |
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