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| 六斑平颜蚜蝇雄虫对雌性腺化合物的触角电位和行为反应 |
| Electroantennogram and behavioral responses of male Eumerus seximaculatum to female gonadal compound（Diptera: Syrphidae） |
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| DOI： |
| 中文关键词: 性信息素 蚜蝇科 平颜蚜蝇属 GC-MS EAG Y型嗅觉仪 |
| 英文关键词:Sex pheromone Syrphidae Eumerus GC-MS EAG Y-tube olfactometer |
| 基金项目:内蒙古自治区关键技术攻关项目（2021GG0057）；中央引导地方科技发展资金项目（2020ZY0001）；内蒙古自然科学基金面上项目（2020MS03037） |
| Author Name | Affiliation | | LI Yan-Ru, SHI Li, HAN Hai-Bin, LIU Ai-Ping, CHEN Guo-Ze | 1. College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010011, China
2. Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, China
3. Mandra Sumu Agriculture and Animal Husbandry Comprehensive Security and Technology Extension Center of Alxa Right Banner, Alxa League 737313, China |
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| 中文摘要: |
| 初步探究肉苁蓉Cistanche deserticola蛀茎蝇—六斑平颜蚜蝇Eumerus seximaculatum雌虫性腺化合物的主要成分并对其生物活性进行检测，为六斑平颜蚜蝇的生物防治提供理论依据。本研究采用气相色谱-质谱联用（GC-MS）技术对雌性腺化合物进行分析鉴定，根据雄成虫的触角电位（EAG）及行为反应对各组分进行筛选、分析及确定。在雌性腺化合物中共鉴定出31种挥发性物质，从中选取5种，设置5个浓度梯度，进行EAG测试。结果表明，雄成虫对5种物质不同浓度的EAG反应敏感性差异明显。其中，雄成虫对1 μg/mL 1,4-丁二醇二甲基丙烯酸酯、100 μg/mL邻苯二甲酸二异丁酯最为敏感；把对雄虫引诱率最高的1,4-丁二醇二甲基丙烯酸酯和邻苯二甲酸二异丁酯按照不同体积比混合后开展行为选择试验，结果显示，当两者比例为1∶3时，对雄虫的引诱率最高，可达60%以上。综上所述，1,4-丁二醇二甲基丙烯酸酯和邻苯二甲酸二异丁酯被认为是雌虫性信息素的重要成分，对雄虫有明显的引诱作用，为苁蓉蛀茎蝇性诱剂的开发奠定理论基础。 |
| 英文摘要: |
| Preliminary explore the main components and identify for its biological activity of the sex pheromone gland of female Eumerus seximaculatum. Provide a theoretical basis for the biological control of E.seximaculatum. In this study, gas chromatography-mass spectrometry (GC-MS) was used to analyze and identify the components of the gonadal compound of female flies. The components were screened, analyzed and determined according by the Electroantennogram (EAG) response and laboratory behavioral response of males. The results showed that a total of 31 chemical substances were identified in the gonadal compound of female flies. Five of them were chosen and five concentration gradients, as well as a control (0.01, 0.1, 1, 10 and 100 μg/mL) were set by EAG test. The results showed that there were significant differences in the males of EAG sensitivity to different concentrations of the five chemicals. Two of them were the most sensitive and the sensitive concentrations were 1 μg/mL 1,4-Butanediol dimethacrylate, 100 μg/mL Diisobutyl phthalate. The experiments of behavioral responses selection were carried out after the 1,4-Butanediol dimethacrylate acid and Diisobutyl phthalate with the different ratios of highest attraction rates to male. The results showed that with the greatest attraction effect to males at the ratio of 1∶3, which could reach more than 60%. In summary, 1,4-Butanediol dimethacrylate and Diisobutyl phthalatewere most likely the important components of sex pheromone of females and had obvious attractant effect to males. A theoretical foundation for the development of sex attractant for the stem-boring flies of Cistanche deserticola. |
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