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| 腐生培养下爪哇虫草对寄主的转录组响应 |
| Transcriptome analysis of Cordyceps javanica in response to host insect in saprophytic culture |
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| DOI： |
| 中文关键词: 爪哇虫草 红火蚁 转录组 腐生培养 弃尸堆 |
| 英文关键词:Cordyceps javanica Solenopsis invicta RNA-seq saprophytic culture refuse piles |
| 基金项目:国家重点研发计划项目（2021YFD1000500）；深圳技师学院校科研项目（2214007，2311008）；广东省农业科学院农业优势产业学科团队建设项目（202117TD） |
| Author Name | Affiliation | | GUAN Zhao-Ying, LEI Yan-Yuan, SUN Xi, FU Zhan-Yuan, HUSSIN Abid | 1. School of Applied Biology, Shenzhen Institute of Technology, Shenzhen 518116, China
2. Guangdong Provincial Key Laboratory of High Technology for Plant Protection/Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
3. College of Agricultural and Food Sciences, King Faisal University, Hofuf 31982, Saudi Arabia |
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| 中文摘要: |
| 爪哇虫草菌是广谱性虫生真菌，具有防治膜翅目社会性昆虫红火蚁Solenopsis invicta的生防潜能。为探究蚁巢弃尸堆中携菌虫体在侵染循环链的关键作用，探讨腐生条件下爪哇虫草Cordyceps javanica响应寄主的分子致病机制，本研究通过向培养基添加冷冻干燥的虫尸粉进行诱导，对诱导前后的菌丝孢子混合体进行转录组测序分析。结果表明，与纯培养条件相比，菌株经虫尸粉诱导后发掘新基因1 912个，有379个得到功能注释。显著差异表达基因有242个，上调表达基因111个，下调表达基因131个；GO富集分析表明，生物学过程的代谢过程和细胞过程、细胞组分的细胞结构体及分子功能中参与催化活性和结合相关的基因可能在爪哇虫草响应寄主过程中发挥了重要作用；KEGG分类和富集分析表明，代谢过程分类的色氨酸代谢、缬氨酸、亮氨酸和异亮氨酸降解、糖酵解/糖原异生和乙醛酸和二羧酸酯代谢，遗传信息过程分类的碱基切除修复和内质网的蛋白质加工，环境信息过程分类的ABC转运蛋白和丝裂原活化蛋白激酶信号通路，细胞过程分类的自噬和过氧物酶体通路是爪哇虫草的主要代谢途径。随机筛选8个差异表达基因进行实时荧光定量PCR验证，这些基因的表达模式与转录组数据分析结果基本一致。本研究结果表明，爪哇虫草在适应腐生环境过程中与氨基酸代谢和能量代谢相关的基因活跃，这可能是其在垂直和水平扩散过程中需维持侵染力，为自身提供营养并转化为能量和中间产物等物质所致。 |
| 英文摘要: |
| Cordyceps javanica is a broad-spectrum insecticidal fungus with biocontrol potential against the social insect of hymenoptera, Solenopsis invicta. The aim of this study was to determine the key role of infected dead ants in refuse piles during the infection cycle, and investigate the molecular pathogenic mechanism of C. javanica in response to host component in saprobiotic condition. The mixture of mycelia and conidia which induced by freeze-dried bodies of ants culture before and after was analyzed by transcriptome sequencing. Our findings revealed that, compared with pure culture, C. javanica showed 1 912 new genes after induction, in which 379 novel genes were annotated with a putative function. A total of 242 significantly differentially expressed genes (DEGs) were identified between induced and uninduced culture. Among them, 111 DEGs were up-regulated while 131 down-regulated; GO enrichment analysis indicated that metabolic process and cellular process classified as biological process, cellular anatomical entity classified as cellular component, and catalytic activity and binding classified as molecular function might play an important role when C. javanica adapted to saprobiotic condition; KEGG classification analysis showed that tryptophan metabolism, valine, leucine and isoleucine degradation, glycolysis/gluconeogenesis and glyoxylate and dicarboxylate metabolism of metabolism classification, base excision repair and protein processing in endoplasmic reticulum of genetic information processing classification, ABC transporters and MAPK signaling pathway-yeast of environmental information processing classification, autophagy-yeast and peroxisome of cellular processes were the main metabolic pathways. Eight genes were randomly selected to verify the expression trend by quantitative real-time fluorescence PCR. The qRT-PCR results showed that the expression trend was consistent with the results of transcriptome data. In this study, we found that the genes related to the amino acids and energy metabolism were active. The possible reasons is that the needs of maintenance of virulence in vertical and horizontal diffusion of C. javanica, and the use of nutrients converting to energy and intermediates assisted with limited host nutrients. |
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