|
| 不同地区甜菜夜蛾解毒酶活性测定和酶抑制剂的增效作用 |
| Detoxification enzyme activity of Spodoptera exigua (Hübner) in different areas and synergistic effect of enzyme inhibitor |
| |
| DOI： |
| 中文关键词: 甜菜夜蛾 解毒酶 酶抑制剂 增效作用 抗药性 |
| 英文关键词:Spodoptera exigua detoxification enzyme enzyme inhibitor synergistic effect resistance |
| 基金项目:国家科技型中小企业技术创新基金（蔬菜高效低毒农药应用技术研究服务平台） |
| Author Name | Affiliation | | ZHOU Li-Lin, XIONG Kai-Fan, WANG Pan, YANG Fan, WANG Yong, WANG Xiao-Ping, SI Sheng-Yun | 1. Institute of Vegetables, Wuhan Academy of Agricultural Sciences, Wuhan 430345, China
2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China |
|
| Hits: 82 |
| Download times: 150 |
| 中文摘要: |
| 本研究测定了广州白云（BY）、武汉黄陂（HP）、上海崇明（CM）、阜阳界首（JS）4个甜菜夜蛾Spodoptera exigua田间种群和敏感种群多功能氧化酶（MFO）、谷胱甘肽S-转移酶（GST）和羧酸酯酶（CarE）酶活性，发现4个田间种群的3种酶活性中仅MFO酶活性均显著高于敏感种群。采用浸叶法测定了MFO抑制剂胡椒基丁醚（PBO）对BY和HP两个田间种群已产生抗性的药剂的增效作用，结果表明PBO分别与高效氯氰菊酯、甲维盐和氯虫苯甲酰胺混用时，对BY和HP种群均有显著增效作用。进一步以这3种制剂最高推荐用量分别与PBO两个浓度（100 mg/L和50 mg/L）混用进行室内盆栽药效试验，与各自单剂最高推荐用量相比，PBO浓度在100 mg/L和50 mg/L时均对高效氯氰菊酯有显著增效作用，PBO浓度在100 mg/L时对甲维盐和氯虫苯甲酰胺有一定增效作用。本研究结果为制定有效的甜菜夜蛾抗药性治理策略提供了技术支撑。 |
| 英文摘要: |
| The activities of Multifunctional oxidase (MFO), Glutathione S-transferase（GST） and Carboxyl esterase（CarE） in four field (BY, HP, CM and JS) and sensitive populations of Spodoptera exigua were determined. Only the activities of MFO in 3 kinds of detoxification enzymes of the 4 field populations were significantly higher than those in sensitive population. Then the synergistic effect of Piperonyl butoxide (PBO), an inhibitor of MFO, on the resistant insecticides of BY and HP populations was tested by the leaf immersion method respectively. The results showed that PBO had significant synergistic effect on both populations when combined with Beta-cypermethrin, Emamectin benzoate and Chlorantraniliprole respectively. Therefore, the 3 preparations mentioned above at the maximum recommended dosage were mixed with 2 concentrations of PBO (100 mg/L and 50 mg/L) respectively. Compared with the maximum recommended dosage of the 3 preparations, PBO at 100 mg/L and 50 mg/L 〖JP2〗showed the obvious synergistic effects on Beta-cypermethrin, and PBO at 100 mg/L had a certain synergistic effects on Emamectin benzoate and Chlorantraniliprole. The results of this study provided technical support for developing effective strategies for the management of insecticide resistance in S.exigua. |
| View Full Text View/Add Comment Download reader |
| Close |
|
|
|
About Journal