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| 毛足棒角蝗对高效氯氰菊酯、印楝素和苦参碱的敏感性监测及解毒酶活性分析 |
| Susceptibility monitoring and detoxification enzyme activity analysis of Dasyhippus barbipes (Orthoptera: Acridoidea) to beta-cypermethrin, azadirachtin and matrine |
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| DOI： |
| 中文关键词: 毛足棒角蝗 抗药性 解毒酶活性 相关分析 通径分析 |
| 英文关键词:Dasyhippus barbipes insecticide resistance detoxification enzyme activity correlation analysis path analysis |
| 基金项目:内蒙古自治区自然科学基金（2021MS03021）；国家重点研发计划（2022YFD1401102）；国家科技基础资源调查专项（2019FY100400） |
| Author Name | Affiliation | | GAO Hai-Yan, YANG Xu-Bing, YAN Feng, SUN Yan-Nan, HAN Hai-Bin, GAO Shu-Jing | 1. Grassland Research Institute of Chinese Academy of Agricultural Sciences, Hohhot 010010, China
2. Ordos Forestry and Grassland Enterprise Development Center, Ordos 017010, Inner Mongolia Automonous Region,China |
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| 中文摘要: |
| 为明确毛足棒角蝗Dasyhippus barbipes田间种群对高效氯氰菊酯、印楝素和苦参碱的药物敏感性现状及代谢抗性机制。采用点滴法测定了四子王旗、锡林浩特、正镶白旗、扎鲁特旗和新巴尔虎左旗蝗虫高发地区毛足棒角蝗3龄蝗蝻对3种杀虫剂的抗药性；检测了不同田间种群毛足棒角蝗体内羧酸酯酶（Carboxylesterase，CarE）、谷胱甘肽-S-转移酶（Glutathione-S-transferase，GST）、多功能氧化酶（Multifunctional oxidase，MFO）和细胞色素P450（CytochromeP450，CYP450）的活性，并分析了毛足棒角蝗的抗性水平与不同解毒酶活性之间的相关性。结果表明：毛足棒角蝗5个种群对高效氯氰菊酯敏感性明显降低，3龄蝗蝻的抗性倍数依次为5.91倍、5.28倍、4.69倍、3.18倍和4.06倍；四子王旗、正镶白旗和扎鲁特旗种群对苦参碱敏感性变化明显，5个毛足棒角蝗种群对印楝素敏感性无明显变化。不同田间种群的解毒酶活性均显著高于敏感种群，其中CarE、GST和CYP450活性与敏感种群差异显著，MFO活性升高不显著。相关分析表明，毛足棒角蝗体内的GST、CYP450活性对印楝素和苦参碱抗性水平存在正相关，相关系数分别为0.672、0.533和0.891、0.845；CarE、GST酶活性对高效氯氰菊酯抗性水平存在极显著正相关，相关系数为0.908和0.842，CYP450酶活性对高效氯氰菊酯抗性水平存在显著正相关，相关系数为0.704。通径分析表明，GST、CYP450活性对印楝素和苦参碱抗性起正向效应，CarE、MFO起间接效应；CarE、GST对高效氯氰菊酯抗性呈现正向效应，发挥直接作用，CYP450则发挥间接作用。毛足棒角蝗的解毒酶与抗药性水平关系密切，多种解毒酶协同发挥作用可降低杀虫剂的毒力，在抗性发展中起到了促进作用，为草原蝗虫治理科学选用杀虫剂、提高防治效率提供科学依据。 |
| 英文摘要: |
| In order to clarify the insecticide sensitivity status and metabolic resistance mechanism of Dasyhippus barbipes field population to beta-cypermethrin, azadirachtin and matrine. The resistance of the 3rd instar nymphs of D.barbipes to three insecticides was determined by drop method in Siziwang Banner(SB), Xilinhot City(XC), Zhengxiangbai Banner(BQ), Zalut Banner(ZB) and Xinbaerhu Left Banner(XB). The activities of Carboxylesterase(CarE), Glutathione-S-transferase(GST), Multifunctional oxidase(MFO) and Cytochrome P450(CYP450) in different field populations of D.barbipes were detected, and the correlation between the resistance level and the activities of different detoxification enzymes was analyzed. The results showed that the sensitivity of five D.barbipes populations to beta-cypermethrin was significantly reduced, and the resistance ratios of 3rd instar nymphs were 5.91, 5.28, 4.69, 3.18 and 4.06 fold, respectively. The populations of Siziwang Banner, Zhengxiangbai Banner and Zarut Banner to matrine changed significantly, and the sensitivity of five D.barbipes populations in five regions to azadirachtin did not change significantly. The activities of detoxification enzymes in different field populations were significantly higher than those in Sensitive populations, and CarE, GST and CYP450 activities were significantly different from those in Sensitive populations, while MFO activity was not significantly increased. Correlation analysis showed that GST and CYP450 activities were positively correlated with azadirachtin and matrine resistance levels, with correlation coefficients of 0.672, 0.533 and 0.891, 0.845, respectively. CarE and GST activities were significantly positively correlated with beta-cypermethrin resistance, with correlation coefficients of 0.908 and 0.842, respectively. CYP450 activity was significantly positively correlated with beta-cypermethrin, and the correlation coefficient was 0.704. Path analysis showed that GST and CYP450 activities had positive effects on azadirachtin and matrine resistance, and CarE and MFO had indirect effects. CarE and GST showed positive and direct effects on beta-cypermethrin resistance, while CYP450 played an indirect role. The detoxifying enzymes of D.barbipes were closely related to the resistance level of insecticides. The synergistic effect of various detoxifying enzymes could reduce the toxicity of insecticides and promote the development of resistance. It provides a scientific basis for the scientific selection of insecticides and the improvement of control efficiency for grassland locust control. |
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