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| 管氏肿腿蜂寄生和注射其毒液对黄粉虫过氧化氢酶基因表达的影响 |
| Effects of parasitization by Scleroderma guani and injection of its venom on the expression of catalase gene in Tenebrio molitor |
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| DOI： |
| 中文关键词: 寄生蜂 毒液 过氧化氢酶 表达特征 酶活性 |
| 英文关键词:Parasitoid venom catalase expression pattern enzyme activity |
| 基金项目:国家自然科学基金（32060126，31660629）；国家林草科技创新计划青年拔尖人才项目（2019132615）；云南省高层次人才培养项目（YNWR-QNBJ-2018-393）；中国科学院“西部之光”人才培养计划“西部青年学者”项目 |
| Author Name | Affiliation | | LIN Ting-Nan, ZHAO Man, WU Chao-Yan, WANG Yu-Qin, WANG Jun, ZHU Jia-Ying | 1. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China
2. Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China |
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| 中文摘要: |
| 为了探究管氏肿腿蜂Scleroderma guani寄生和注射其毒液对寄主黄粉虫Tenebrio molitor蛹中过氧化氢酶表达的影响，采用RT-PCR克隆黄粉虫过氧化氢酶基因，利用生物信息学软件分析基因序列结构特性，采用实时荧光定量PCR技术分析该基因的表达特征，使用试剂盒测定过氧化氢酶活性。克隆获得的黄粉虫过氧化氢酶基因编码阅读框序列长1 620 bp，编码539个氨基酸。该基因编码的氨基酸序列中含有催化氨基酸位点（His-71、Asn-183和Tyr-393）以及过氧化氢酶家族的3个保守基序：近端活性位点序列（FDRERIPERVVHAKGXG）、NADPH结合位点（XXHQXXXXFXD）和血红素配体结合位点（RXFXYXDXH）。被管氏肿腿蜂寄生和注射其毒液后，黄粉虫蛹中的过氧化氢酶基因的表达量显著上调，其血淋巴和脂肪体中的过氧化氢酶活性显著升高。研究结果表明，管氏肿腿蜂毒液能调控黄粉虫过氧化氢酶基因的表达。 |
| 英文摘要: |
| In order to investigate the effects of parasitization by Scleroderma guani and injection of its venom on the expression of catalase gene in Tenebrio molitor pupae. The catalase gene of T. molitor was cloned by RT-PCR. Its sequence characteristics were analyzed by bioinformatics software. Real-time fluorescence quantitative PCR was used to analyze the gene expression patterns of catalase gene. The enzyme activity of the catalase was determined with the kit. The cloned open reading frame of the catalase gene of T. Molitor was 1 620 bp in length, encoding 539 amino acids. There are catalytic amino acids (His-71, Asn-183, Tyr-393) and three conserved motifs present in the amino acid sequence encoding by this gene, proximal active site signature (FDRERIPERVVHAKGXG), NADPH binding site (XXHQXXXXFXD) and proximal heme-ligand signature (RXFXYXDXH). After parasitization by S.guani and injection of its venom, the gene expression level of catalase in pupae was significantly up-regulated, and the catalase activity in the hemolymph and fat body of the T. molitor pupae was significantly increased. The results indicate that venom of S. guani participates in regulating the expression of catalase in T. molitor. |
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