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| 利用透射电镜观察破坏素A对烟粉虱的组织病理影响 |
| Observation of histopathological effects of destruxin A on Bemisia tabaci by transmission electron microscope |
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| DOI： |
| 中文关键词: 烟粉虱 破坏素A 透射电镜 |
| 英文关键词:Bemisia tabaci destruxin A TEM |
| 基金项目:广州市科技计划（202102021290）；广东省基础与应用基础研究基金（2022A1515110253）；广东省普通高校创新团队项目（2022KCXTD050） |
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| 中文摘要: |
| 为了更好地了解破坏素A对烟粉虱Bemisia tabaci（Gennadius）（半翅目Hemiptera粉虱科Aleyrodidae）的作用机理，采用透射电镜技术观察饲喂破坏素A后烟粉虱成虫中肠、脂肪体等组织的病理学变化。结果表明：取食破坏素A后，烟粉虱成虫的中肠细胞受到了严重损坏，处理早期主要体现在细胞空泡化严重，核膜膨胀，而在处理后期，所有微绒毛脱落，细胞器等内容物被消化。烟粉虱成虫脂肪体细胞在破坏素A处理早期颜色变浅，脂滴膜结构变透明，出现大量空泡，在处理后期脂滴膜结构崩解。中肠和脂肪体细胞崩解可能是破坏素A致死烟粉虱的重要原因之一，可为进一步研究破坏素A的作用机制提供科学依据。 |
| 英文摘要: |
| To better understand the mechanism of action of destruxin A (DA) on Bemisia tabaci (Gennadius) (Hemiptera∶Aleyrodidae), transmission electron microscopy (TEM) was used to observe the pathological changes in the midgut and fat body tissues of adult whiteflies fed with DA. The results showed that the midgut cells of the whiteflies were severely damaged after feeding with DA. The vacuolization of the cells accrued and the nuclear membrane were swelling in the early treatment stage. At the later stage of treatment, all the microvilli fell off and the contents of the organelles died out. The fat body cells of B. tabaci adults became lighter in color in the early stage of destruxin A treatment and the lipid droplet membrane structure became transparent. Moreover, a large number of vacuoles appeared. At the later stage of treatment, the lipid droplet membrane structure collapsed. The breakdown of midgut and fat body cells may be one of the important reasons for the death of whiteflies caused by DA. Our results providing a scientific basis for further research into the mechanism of DA. |
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