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| 捕食性蝽类唾液腺解剖方法—以叉角厉蝽为例 |
| A protocol for dissecting the salivary gland from predatory bug—A case study in Eocanthecona furcellata |
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| DOI： |
| 中文关键词: 捕食性蝽 叉角厉蝽 唾液腺 解剖 形态结构 |
| 英文关键词:Predatory bug Eocanthecona furcellata salivary gland dissection morphology |
| 基金项目:云南省农业基础研究联合专项重点项目（202101BD070001-024）；云南省高层次人才培养项目（YNWR-QNBJ-2018-393） |
| Author Name | Affiliation | | SU Ding-Yi, WANG Yu-Qin, LI Lu, WU Guo-Xing, ZHU Jia-Ying | 1. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China
2. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China |
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| 中文摘要: |
| 唾液腺的解剖是研究捕食性蝽类唾液成分及功能的关键。以叉角厉蝽Eocanthecona furcellata为例，报道了解剖获得其完整唾液腺的方法。该方法与其他解剖方法相比明显不同之处在于使用镊子固定虫体，更有利于唾液腺的解剖与收集。通过拍照观察，叉角厉蝽的唾液腺由主腺、副腺、肺门和导管组成。主腺可分为主腺前叶和主腺后叶，它们之间通过肺门相连。主腺肺门处着生有两根导管，其中一根导管与口器相连，而另一根导管与副腺连接。本文描述的解剖方法以及叉角厉蝽唾液腺形态结构可用于指导解剖获得其它捕食性蝽类的唾液腺，进而提取唾液开展成分鉴定和功能研究。 |
| 英文摘要: |
| Dissection of the salivary gland is key to identify the components of saliva from predatory bug and investigate its functions. In this work, a case study was carried out in a predatory bug, Eocanthecona furcellata. A protocol for dissecting the complete salivary gland from this bug was described. In composition with other dissection methods, the distinct aspect of this method was using the tweezers to fix the insect body, which was helpful for dissecting and collecting the salivary gland. According to its morphology, the salivary gland of E. furcellata was composed by main gland, accessory gland, hilum and duct. Main gland can be divided into anterior main gland and posterior main gland. There was a duct connected the main gland and mouthpart, and another duct connect the main gland and accessory gland, of which both ducts were connected to hilum in the main gland. The protocol reported in this study and morphology of the salivary gland of E. furcellata can be used to guide the dissection of salivary gland from other predatory bugs to extract saliva for deciphering its components and characterizing its functions. |
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