| Tuta absoluta is a world-destructive tomato pest. In order to clarify the composition of culturable gut bacteria in the larvae, Luria-Bertani and Nutrient Agar media were used to isolate and culture the gut bacteria of T. absoluta larvae in this study, and culturable bacteria were identified according to colony morphology and 16S rDNA sequence analysis. Turbidimetric method was used to determine the growth curve of dominant species, and transparent circle method was used to determine the degradation ability of starch and cellulose by culturable gut bacteria. The results showed that there were 27 strains were isolated from the gut of the 3th instar larvae of T. absoluta, which belonging to 24 species, 17 genuses, 10 families and 3 phyla, among which dominant phylum, family, genus and species were Proteobacteria, Erwiniaceae, Erwinia and Erwinia iniecta with relative abundances of 90.68%, 89.41%, 89.41% and 89.41%, respectively. The dominant species, E.iniecta, had no lag period at 25℃ and 180 r/min, it’s logarithmic growth period was 0~14 h, the stable period was 14~28 h, and the decline period was after 28 h. Starch and cellulose could be degraded by L7 and L9 of Glutamicibacter, L14 of Kocuria and L20 of Brachybacterium, while L15 and L17 of Kocuria could only degrade starch. L of Erwinia, L11 of Planococcus, L18 of Microbacterium and L22 of Prolinoborus could only degrade cellulose, while other strains had no ability of starch and cellulose degradation. These results indicated that 24 kinds of gut culturable bacteria which were contained by T. absoluta larvae and it’s abundant in species, starch and cellulose could be degraded by several bacteria. Collectively, the results will provide evidence for the further study of gut bacterial diversity and function of T. absoluta, and also culturable strains for the development and utilization of functional bacteria can be provided. |
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