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| 几种土壤真菌对烟粉虱的生物活性研究 |
| Bioactive of soil fungi against whitefly |
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| DOI： |
| 中文关键词: 土壤真菌 淡紫紫孢菌 刀孢蜡蚧菌 烟粉虱 |
| 英文关键词:Soil fungi Purpureocillium lilacinum Lecanicillium psalliotae Bemisia tabaci bioactivity |
| 基金项目:广州市科技计划项目（201903010067） |
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| 中文摘要: |
| B型烟粉虱Bemisia tabaci是重大入侵害虫，发展生物防治技术控制其危害具有重要意义。本研究采用浸渍法测定了9种20株土壤真菌对B型烟粉虱的室内活性，结果表明，供试菌株对B型烟粉虱都有一定致病活性，1.0±10^8 孢子/mL的分生孢子悬浮液处理10 d后，烟粉虱2龄若虫的累计校正死亡率为53%~93%，其中淡紫紫孢菌Purpureocillium lilacinum PITS01、PlMS-12和PlMS-15菌株，以及刀孢蜡蚧菌Lecanicillium psalliotae LpTS01菌株引起B型烟粉虱91%以上的死亡率。进一步测定了PITS01和LpTS01菌株对烟粉虱的毒力，结果表明PITS01和LpTS01对烟粉虱2龄若虫在处理第4天的LC50分别为2.8×10^7 孢子/mL和3.6×10^7 孢子/mL，第6天分别为1.7×10^6 孢子/mL和3.6×10^5 孢子/mL；当孢子浓度为1.0×10^7 孢子/mL时，PITS01和LpTS01菌株LT50值分别为4.4 d和4.2 d。菌株PITS01和LpTS01对烟粉虱防治具有一定的应用潜力。 |
| 英文摘要: |
| The B-biotype of whitefly (Bemisia tabaci) is an important invasion pest. It is significant to develop biocontrol technology to overcome the pest damage. In current study, the bioactivities of 20 fungal strains in 9 species from soil against whitefly nymph were evaluated by means of immersion method. The results indicated that all the strains had certain pathogenicity to B. tabaci. After treated with 1.0×10^8 spores/mL for 10 days, the calibrated mortality of the 2nd instar nymphs of B. tabaci was 53%~93%. Among them, the three strains of Purpureocillium lilacinum PITS01, BbTs03, AuTS01 and the Lecanicillium psalliotae LpTS01 strain had higher bioactivity with whiteflies’ mortality > 91%. Furthermore, the experiments of virulence tests indicated that the LC50 of PITS01 and LpTS01 to the 2nd instar nymph of B. tabaci were 2.8×10^7 spores/mL and 3.6×10^7 spores/mL on the 4th day after treatment, and 1.7×10^6 spores/mL and 3.6×10^5 spores/mL on the 6th day after treatment, respectively. Meanwhile, the LT50 values of PITS01 and LpTS01 at 1.0×10^7 spores/mL of the concentration, were respectively recorded as 4.4 d and 4.2 d. Obviously, the results suggested that the strains PITS01 and LpTS01 had the potential for whitefly biocontrol. |
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