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| 管纹艳虎天牛糖苷水解酶基因家族的鉴定和表达谱分析 |
| Identification and expression profile analysis of glycoside hydrolase gene families in Rhaphuma horsfieldi |
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| DOI： |
| 中文关键词: 管纹艳虎天牛 糖苷水解酶 进化分析 同源建模 表达谱 |
| 英文关键词:Rhaphuma horsfieldi glycoside hydrolase phylogenetic analysis homology modeling expression profile |
| 基金项目:云南省教育厅科学研究基金项目（2019Y0141，2020Y398）；云南省“高层次人才培养支持计划”青年拔尖人才项目（YNWR-QNBJ-2019-057） |
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| 中文摘要: |
| 昆虫糖苷水解酶（Glycoside hydrolase, GH）在寄主植物糖类化合物的水解过程中扮演着重要作用，但是在管纹艳虎天牛Rhaphuma horsfieldi中尚未有GH基因的报道。基于测序的转录组数据，本研究从管纹艳虎天牛中鉴定到7个RhorGHs家族：GH1、GH9、GH13、GH16、GH28、GH31和GH45，分别具有23、1、4、2、10、12和4个基因，其中23个基因具有全长序列。在进化分析中，管纹艳虎天牛不同GH家族的成员主要以物种特异的形式聚类，尤其是GH1和GH28家族。三级结构分析显示，RhorGH28家族的蛋白主要由β折叠组成，不同蛋白间具有高度保守的结构和与糖类化合物互作的关键氨基酸位点，包括质子供体、催化亲核残基和底物结合位点。在表达谱分析中，大部分RhorGHs基因主要在雌雄虫腹部特异或高表达，暗示其可能在与消化有关的肠道中表达。研究结果明确了管纹艳虎天牛GH家族及其数量，以及各家族的序列特征和进化关系，可为该天牛的寄主植物适应性机制研究提供借鉴。 |
| 英文摘要: |
| Glycoside hydrolase (GH) in insects is of particular importance for the hydrolysis of carbohydrate polymers, but little is known about this gene family in Rhaphuma horsfieldi. Based on the transcriptome of R. horsfieldi, totally seven gene families of RhorGHs were identified, representing GH1, GH9, GH13, GH16, GH28, GH31 and GH45 with 23, 1, 4, 2, 10, 12 and 4 relatives, respectively. Among them, 23 genes were predicted to have complete open reading frames. Phylogenetic analysis indicated that RhorGHs of each gene family clustered together in species-specific expansions, especially for GH1 and GH28 families. Tertiary structural analysis revealed that RhorGH28 proteins were mainly composed of β-sheets, and had highly conserved structure and key amino acids interacting with carbohydrates, such as the proton donors, catalytic nucleophiles and substrate binding residues. In the expression profiling analyses, most of RhorGHs were specifically or highly expressed in female or male abdomens, suggesting their presence in guts involving digestive functions. Taken together, this study has identified RhorGH gene families, as well as their numbers, sequence characteristics and phylogenetic relationships in R. horsfieldi, and facilitates further studies on adaptive mechanisms of this beetle to host plants. |
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