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| 新疆地区绿僵菌菌株筛选及抗逆性研究 |
| Screening and stress resistance of Metarhizium strains in Xinjiang |
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| DOI： |
| 中文关键词: 绿僵菌 高毒力 东亚飞蝗 抗逆性 |
| 英文关键词:Metarhizium high toxicity Locusta migratoria manilensis stress resistance |
| 基金项目:新疆特殊环境物种多样性应用与调控重点实验室；国家自然科学基金（31360088）；新疆维吾尔自治区“天山青年计划”(2017Q024) |
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| 中文摘要: |
| 本文对新疆地区土壤中分离得到的17株绿僵菌Metarhizium菌株以东亚飞蝗Locusta migratoria manilensis为供试昆虫进行毒力测定，筛选获得高毒力菌株，并对筛选后的高毒力绿僵菌菌株进行耐短时高温能力、抗紫外线能力和耐干旱能力的测试，分析高毒力绿僵菌菌株的抗逆性，以期获得致病力高且抗逆性好的菌株，为下一步绿僵菌生物农药的开发提供依据。研究发现，M1-17、M1-13、M1-09、M1-16、M1-05五株菌株为对东亚飞蝗高致病力的菌株，平均僵虫率在80.00%~96.67%之间，LT50在2.92~3.65之间。对高温的抗性效果较好的菌株为M1-17和M1-05；对紫外线的抗性效果较好的菌株为M1-17和M1-16；而菌株M1-09、M1-17和M1-16抗旱能力较好。菌株M1-17较其它菌株具有更好的抗逆性，具有很好的开发利用价值。 |
| 英文摘要: |
| This paper carried out a toxicity test on the 17 Metarhizium strains isolated from soil in Xinjiang, with Asiatic migratory locust as the test material. High-toxicity strains were screened. In order to obtain high-toxicity and resistance strains as well as provide a basis for the subsequent development of Metarhizium biological pesticides, the short-term high temperature resistance, UV resistance and drought resistance of the high-toxicity Metarhizium strains screened were tested to analyze the resistance of these strains. The study showed that, the M1-17, M1-13, M1-09, M1-16 and M1-05 strains were highly pathogenic to Asiatic migratory locust, with a cadaver rate between 80.00%~96.67% and LT50 between 2.92~3.65. Strains M1-17 and M1-05 had good resistance to high temperature; strains M1-17 and M1-16 had good resistance to ultraviolet light; and strains M1-09, M1-17 and M1-16 had good drought resistance. Strain M1-17 showed better resistance than other strains, making it highly significant for development and utilization. |
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