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| 家蚕Bmlark基因启动子活性及转录调控元件分析 |
| Activity and transcriptional regulatory elements of the promoter in Bmlark gene |
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| DOI： |
| 中文关键词: Bmlark基因 启动子 双萤光素酶报告系统 结合蛋白 |
| 英文关键词:Bmlark gene promoter dual luciferase reporter system binding protein |
| 基金项目:国家自然科学基金（31720103916，31930102，32000337） |
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| 中文摘要: |
| LARK蛋白是一个参与多个发育途径的重要调控转录因子。为研究家蚕Bmlark基因启动子活性及其调控蛋白，本研究克隆了家蚕Bmlark基因的启动子序列（-1 232~ +211），构建了该启动子萤光素酶报告质粒，转染至家蚕Bm12细胞中，进行启动子萤光素酶活性检测。结果表明，Bmlark基因的-124~ -92 bp和-220~-124 bp区域参与了其转录调控。利用-124~ -92 bp区域的DNA片段作为分子探针，发现该区域可能与蛋白TAF7，Ets transcription factor，protein abrupt isoform X1和forkhead box protein N3等结合。研究结果为进一步深入研究Bmlark转录调控的分子机制提供了线索。 |
| 英文摘要: |
| LARK protein is an important regulatory transcription factor involved in multiple developmental pathways. In order to study the activity of the promoter of the silkworm Bmlark gene and its regulatory protein, this study cloned the promoter sequence of the Bmlark gene of the silkworm (-1 232~ +211) to construct the luciferase reporter plasmid of the promoter and transfected it into the silkworm Bm12 cells. Promoter luciferase activity was tested, and the results showed that the-124~ -92 bp and-220~ -124 bp regions of the Bmlark gene were involved in its transcriptional regulation. Using the DNA fragment of-124~ -92 bp region as a molecular probe, it was found that this region may bind to protein TAF7, Ets transcription factor, protein abrupt isoform X1 and forkhead box protein N3. The research results provided clues for further in-depth study of the molecular mechanism of Bmlark transcription regulation. |
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