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| 蛋白质组学揭示美洲大蠊卵鞘在胚胎发育中的重要功能 |
| Proteome profiling reveals oothecal function during embryogenesis in the American cockroach, Periplaneta americana |
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| DOI： |
| 中文关键词: 美洲大蠊 卵鞘 蛋白组 label-free 富集分析 |
| 英文关键词:Periplaneta americana ootheca proteome label-free enrichment analysis |
| 基金项目:国家自然科学基金(3172021) |
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| 中文摘要: |
| 作为世界性的卫生害虫，美洲大蠊 Periplaneta americana 对环境有惊人的适应性和极强的繁殖力，其强大的繁殖能力以及卵鞘对胚胎发育的有效保护，是其防治困难的内在原因。本研究以美洲大蠊卵鞘为研究对象，利用Label-free定量技术提取前期和中期的美洲大蠊卵鞘蛋白，检验合格后的蛋白溶液通过酶解，液质联用检测技术后，利用MaxQuant软件对下机数据进行蛋白质鉴定和label-free定量分析，对差异蛋白进行GO功能注释和KEGG代谢通路富集分析，并讨论不同发育时期美洲大蠊蛋白质组的表达差异。结果显示，在美洲大蠊前期和中期卵鞘中共鉴定出754个蛋白，其中总差异蛋白220个，157个存在显著性差异，与新鲜卵鞘相比，中期卵鞘中表达量上调的蛋白102个，下调的55个。这些差异蛋白主要富集在生物代谢通路、蛋白质合成、胞吐、黏蛋白类、蛋白合成与降解、免疫等过程，它们与卵鞘的形成，胚胎发育时期的保水、免疫等功能密切相关。本研究为进一步研究美洲大蠊卵鞘功能奠定了基础，为美洲大蠊的生物防治提供科学依据。 |
| 英文摘要: |
| Periplaneta americana, as a world-wide sanitary pest, has amazing adaptability to the environment and strong fecundity. Its strong reproductive ability and ootheca occurrence are the internal reasons against pest control. In this study, the non-labeled quantitative proteomics were employed to compare the differential proteins of oothecae between D1 and D20. Briefly, extracted the proteins and detected them by HPLC/MS technology. After identified by MaxQuant software and label-free quantitative analysis, the differential proteins were further performed with GO function annotation and KEGG metabolic pathway enrichment analysis. The results showed that total of 754 proteins were identified, of which 157 proteins are significantly different. Among them, there were 102 up-regulated proteins and 55 down-regulated proteins. These differential proteins were mainly enriched in immunity, water retention, ootheca formation, ect. These data would provide a scientific basis for both ootheca function and the biological control of American cockroach. |
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