The composition and diversity of the endophytic yeast species in Vespa velutina Smith were studied by using high-throughput sequencing based on 26S rDNA and diluted plate method in Shihezi West Park, Xinjiang. The results showed that there were ten genus including Cutaneotrichosporon, Torulaspora, etc. There were ten species of endophytic yeast including Cutaneotrichosporon cutaneum, Torulaspora delbrueckii, Sporidiobolales sp., etc. Shannon index, Simpson index, ACE index and Chao 1 Index are 0.619215,0.681265,84.38456 and 76 respectively. A strain of endophytic yeast, Metschnikowia pulcherrima, was isolated by using diluted plate method combined with colony and cell morphology observation, physiological and biochemical detection and 26S rDNA molecular identification. This study can lay the foundation for subsequent study on endophytic yeast function and utilization of yeast resources. |