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柑橘木虱对12种寄主植物嫩梢及其挥发物成分的趋性反应 |
Tendency response of Diaphorina citri Kuwayama to shoots and volatile components of twelve host plants |
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DOI: |
中文关键词: 柑橘木虱 寄主植物 挥发物 趋性反应 |
英文关键词:Asian citrus psyllid host plant volatiles tendency response |
基金项目:广西科技重大专项(桂科AA18118046) |
Author Name | Affiliation | TANG Xia-An, LI Xian-Liang, CHI Zhang-Chen,WEI Shu-Wei, DENG Ye-Cheng, DENG Zhi-Yong, LUO Hai-Yu, DENG Min-Xue | 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education of China/ College of Life Science, Guangxi Normal University, Guangxi Provice,Guilin 541004, China 2. Guangxi Academy of Specialty Crops, Guangxi Provice,Guilin 541004, China |
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中文摘要: |
为了发现对柑橘木虱 Diaphorina citri Kuwayama具有引诱活性的寄主植物嫩梢挥发物,采用Y型嗅觉仪在室内测定柑橘木虱对12种寄主植物嫩梢及其挥发物成分的趋性反应,采用固相微萃取技术(SPME)和气-质联用分析技术(GC-MS)分析鉴定嫩梢挥发物的共同成分。结果表明,柑橘木虱对九里香 Murraya exotica 、沙田柚 Citus maxima ‘Shatian You’、红肉酸柚 Cgrandis ‘Hongrousuan You’、HB柚 Citus grandis ‘HB’、高斑柚 Citus grandis‘gaoban You’和纽荷尔脐橙Citus sinensis‘Newhall’ 6种植物嫩梢具有显著的趋性反应,选择率分别为66.70%、66.04%、63.77%、63.86%、61.67%和60.35%。从寄主植物嫩梢中鉴定出8种挥发物共同成分,分别为α-石竹烯、(-)-β-石竹烯、萜品油烯、芳樟醇、(+)-α-蒎烯、3-蒈烯、D-柠檬烯和γ-松油烯。柑橘木虱对8种植物挥发物成分均有一定的趋性,其中对浓度为10 μL/mL (-)-β-石竹烯和D-柠檬烯的选择率分别为73.20%和71.88%,对浓度为1 μL/mL芳樟醇的选择率为79.19%。研究结果为进一步开发柑橘木虱引诱剂奠定了基础。 |
英文摘要: |
In order to find the volatile components of shoots of the host plants with attractive activity to citrus psyllid Diaphorina citri Kuwayama, Y-type olfactory instrument was used to measure the tendency response of citrus psyllid to the shoots of twelve host plants and their volatile components, and solid phase microextraction (SPME)and gas-mass spectrometry (GC-MS)analysis were used to identify the common components of volatiles of the shoots. The results showed that the citrus psyllid had significant selective response to the shoots of Murraya exotica , Citrus maxima‘Shatian You’, Citrus grandis ‘Hongrousuan You’, Citrus grandis ‘HB’, Citrus grandis‘gaoban You’and Citrus sinensis ‘Newhall’, and the selective rates were 66.70%, 66.04%, 63.77%, 63.86%, 61.67% and 60.35%, respectively. Eight common volatile constituents were identified from the shoots volatiles of host plants, namely α-humulene, (-)-β-caryophyllene, terpinolene, linalool, ( + )-α-pinene, 3-carene, D-limonene and γ -terpinene. Citrus psyllid has selective response to the 8 plant volatile components. The selective rates to (-)-β-caryophyllene and D-limonene at 10 μL/mL reach 73.2% and 71.88% respectively, and the selective rate to linalool at 1 μL/mL reach 79.19%. The results laid the foundation for further development of attractants of citrus psyllid. |
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