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| 家蝇3种丝氨酸蛋白酶抑制剂( Serpin )基因在感染白色念珠菌后的表达 |
| Expression pattern of three serine protease inhibitor (Serpin) genes after the fungal infection in Musca domestica |
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| DOI： |
| 中文关键词: 丝氨酸蛋白酶抑制剂 家蝇 感染 时空表达 实时荧光定量PCR |
| 英文关键词:Serine protease inhibitor Musca domestica infection spatiotemporal expression patterns real time fluorescent quantitative PCR |
| 基金项目:国家自然科学基金(81360254)；贵州省科技厅省校合作项目(黔科合LH字[2014]7076号)；贵州省卫生计生委科学技术项目（gzwjkj2014-2-100） |
| Author Name | Affiliation | | HU Ya, LUO Man, WEI Chuan-Chuan, XIU Jiang-Fan, WU Jian-Wei | 1. Bijie First Municipal Peoples Hospital, Bijie 551700, Guizhou Province, China
2. Basic Medical College, Medical University of Guizhou, Guiyang 550004, Guizhou Province, China
3. Bijie Hospital of Traditional Chinese Medicine, Bijie 551700, Guizhou Province, China
4. Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, Guizhou Province, China |
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| 中文摘要: |
| 研究家蝇丝氨酸蛋白酶抑制剂（ Serpin ）基因 Sp2、Sp13、Sp16 在感染白色 念珠菌后的表达模式。利用白色念珠菌刺激家蝇幼虫，对收集的标本进行逆转录，以 GAPDH 为内参基因，通过qPCR技术检测3条 Serpin 基因在家蝇感染后不同时间点及不同组织中的表达特征，统计分析结果。结果表明，不同时间点感染组和对照组比较， Sp2 mRNA在3 h、6 h和48 h的表达量比对照组高； Sp13 mRNA在6 h的表达量比对照组高，在3 h和24 h时的表达量比对照组低； Sp16 mRNA在6 h、24 h和48 h时的表达量比对照组高，在36 h时的表达量比对照组低。不同组织中感染组和对照组比较， Sp2 mRNA在3 h时，在血淋巴和脂肪体中的表达量比对照组高，在马氏管、气管和肠道的表达量比对照组低；12 h时，在血淋巴中的表达量比对照组高，在马氏管和唾液腺中的表达量比对照组低；24 h时，在脂肪体中的表达量比对照组低；48 h时，在血淋巴中呈高表达，在唾液腺中的表达量比对照组低。 Sp13 mRNA在3 h时，在唾液腺和脂肪体中的表达量比对照组高，在马氏管、体壁、血淋巴和气管中的表达量比对照组低；12 h时，在血淋巴中呈高表达，在体壁和脂肪体中的表达量比对照组低；24 h时，在血淋巴、气管和唾液腺中的表达量比对照组高，在脂肪体中的表达量比对照组低；48 h时，在马氏管、血淋巴、气管和脂肪体中的表达量比对照组高，在体壁和唾液腺中的表达量比对照组低。 Sp16 mRNA在3 h时，在马氏管和气管中的表达量比对照组低；12 h时，在马氏管和脂肪体中其表达量比对照组低；24 h时，其表达量在马氏管中比对照组高，在脂肪体中比对照组低；48 h时，在血淋巴中呈高表达，而在马氏管中的表达量比对照组低。家蝇3种 Serpin基因在白色念珠菌感染后均呈现出差异性表达，推测 Serpin 基因在家蝇免疫调节中具有协同调节作用。 |
| 英文摘要: |
| To research the expression patterns of serine protease inhibitors (Serpin) genes Sp2, Sp13 and Sp16 in Musca domestica larvae after infection of Canidia Albicans. Musca domestica larvae infection Canidia Albicans and collect specimens, total RNA was extracted from these samples and reverse transcription for cDNA, the expressions of three Serpin genes were detected by qPCR．GAPDH as internal reference gene. Laboratory data were then analysed statistically. Different time points after infection, the results indicated that the expression of Sp2 mRNA in infection group increased obviously in 3 h, 6 h and 48 h compared with control group; The expression of Sp13 in 6 h was higher than the control group, but lower at 3 h and 24 h; The expression of Sp16 in 6 h, 24 h and 48 h was higher than the control group, and the expression of Sp16 in 36 h was lower than the control group. Infection group different tissue compared with control group, the Sp2 mRNA express in the hemolymph and fat body significantly increased in 3 h, but lower expression in the malpighian tubes、trachea and intestinal; Higher expression in the haemolymph, but lower expression in the salivary glands and the malpighian tubes in 12 h; lower expression in the fat body in 24 h; Expressed in the hemolymph quantity higher, but low expression of salivary glands amount than those of the control group in 48 h. The Sp13 mRNA express in the salivary glands and fat body volume significantly increased in 3 h, but lower expression in the malpighian tubes、epidermis、hemolymph and trachea than the control group; Higher expression in the haemolymph, but lower expression in the epidermis and fat body in 12 h; Higher expression in the haemolymph、trachea and the salivary glands, but lower expression in the fat body than the control group in 24 h; Higher expression in the malpighian tubes、haemolymph、trachea and the fat body, but lower expression in the epidermis and salivary glands in 48 h. The Sp16 mRNA is lower expression in the malpighian tubes and trachea than the control group in 3 h; lower expression in the malpighian tubes and fat body in 12 h; Higher expression in the malpighian tubes, but lower expression in the fat body in 24 h; Higher expression in the haemolymph, but lower expression in the malpighian tubes in 48 h. Three Serpin genes of Musca domestica showed differential expression after infection with Candida albicans. It is speculated that Serpin gene has synergistic regulation in the immune regulation of Musca domestica. |
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